Enhanced stability of the cloned Bacillus subtilis alkaline protease gene in alginate-immobilized B. subtilis cells
Expression and stability of the cloned Bacillus subtilis alkaline protease ( aprE)gene was monitored throughout the growth of free and alginate-immobilized B. subtilis cells. The time as well as the level of expression of the aprE gene in alginate-immobilized cells was found to be close to that of f...
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Veröffentlicht in: | Enzyme and microbial technology 2002-06, Vol.30 (7), p.862-866 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Expression and stability of the cloned
Bacillus subtilis alkaline protease (
aprE)gene was monitored throughout the growth of free and alginate-immobilized
B. subtilis cells. The time as well as the level of expression of the
aprE gene in alginate-immobilized cells was found to be close to that of free cells. The multicopy plasmid that carries the
aprE gene was stably maintained in alginate-immobilized cells. Plasmid stability was greatly enhanced, it reached 83% and 8% after ten growth cycles for alginate-immobilized and free cells in the absence of stress, respectively. Data presented demonstrate that immobilization of
B. subtilis recombinant cells would partially solve the problem of plasmid instability in
B. subtilis. |
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ISSN: | 0141-0229 1879-0909 |
DOI: | 10.1016/S0141-0229(02)00046-7 |