Chitosan isolation from the chitosan-glucan complex of fungal cell wall using amylolytic enzymes

The chitosan/glucan complex isolated from the mycelia of the fungus, Gongronella butleri USDB 0201 can be cleaved with a heat-stable α-amylase at 65 °C for 3 h. This results in the removal of the glucan side chain and gives a chitosan solution with 100 times lower turbidity. It is proposed that chit...

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Veröffentlicht in:	Biotechnology letters 2002-09, Vol.24 (18), p.1461-1464
Hauptverfasser:	NWE, Nitar, STEVENS, Willem F
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Cells Enzyme engineering Enzymes Fundamental and applied biological sciences. Psychology Fungi Methods. Procedures. Technologies Miscellaneous Turbidity
Online-Zugang:	Volltext
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Zusammenfassung:	The chitosan/glucan complex isolated from the mycelia of the fungus, Gongronella butleri USDB 0201 can be cleaved with a heat-stable α-amylase at 65 °C for 3 h. This results in the removal of the glucan side chain and gives a chitosan solution with 100 times lower turbidity. It is proposed that chitosan and glucan chains are bound by an α(1 to 4) glucosidic bond. Both fungal chitosan and fungal glucan have been purified separately.[PUBLICATION ABSTRACT]
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1019898715518