Soluble Dietary Fiber Ameliorates Radiation-Induced Intestinal Epithelial-to-Mesenchymal Transition and Fibrosis
Background: Intestinal fibrosis is a late complication of pelvic radiotherapy. Epithelial-to-mesenchymal transition (EMT) plays an important role in tissue fibrosis. The aim of this study was to examine the effect of soluble dietary fiber on radiation-induced intestinal EMT and fibrosis in a mouse m...
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| Veröffentlicht in: | JPEN. Journal of parenteral and enteral nutrition 2017-11, Vol.41 (8), p.1399-1410 |
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| container_title | JPEN. Journal of parenteral and enteral nutrition |
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| creator | Yang, Jianbo Ding, Chao Dai, Xujie Lv, Tengfei Xie, Tingbing Zhang, Tenghui Gao, Wen Gong, Jianfeng Zhu, Weiming Li, Ning Li, Jieshou |
| description | Background: Intestinal fibrosis is a late complication of pelvic radiotherapy. Epithelial-to-mesenchymal transition (EMT) plays an important role in tissue fibrosis. The aim of this study was to examine the effect of soluble dietary fiber on radiation-induced intestinal EMT and fibrosis in a mouse model. Materials and Methods: Apple pectin (4% wt/wt in drinking water) was administered to wild-type and pVillin-Cre-EGFP transgenic mice with intestinal fibrosis induced by a single dose of abdominal irradiation of 10 Gy. The effects of pectin on intestinal EMT and fibrosis, gut microbiota, and short-chain fatty acid (SCFA) concentration were evaluated. Results: Intestinal fibrosis in late radiation enteropathy showed increased submucosal thickness and subepithelial collagen deposition. Enhanced green fluorescent protein (EGFP)+/vimentin+ and EGFP+/α–smooth muscle actin (SMA)+ coexpressing cells were most clearly observed at 2 weeks after irradiation and gradually decreased at 4 and 12 weeks. Pectin significantly attenuated the thickness of submucosa and collagen deposition at 12 weeks (24.3 vs 27.6 µm in the pectin + radiation-treated group compared with radiation-alone group, respectively, P < .05; 69.0% vs 57.1%, P < .001) and ameliorated EMT at 2 and 4 weeks. Pectin also modulated the intestinal microbiota composition and increased the luminal SCFA concentration. Conclusion: The soluble dietary fiber pectin protected the terminal ileum against radiation-induced fibrosis. This effect might be mediated by altered SCFA concentration in the intestinal lumen and reduced EMT in the ileal epithelium. |
| doi_str_mv | 10.1177/0148607116671101 |
| format | Article |
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Epithelial-to-mesenchymal transition (EMT) plays an important role in tissue fibrosis. The aim of this study was to examine the effect of soluble dietary fiber on radiation-induced intestinal EMT and fibrosis in a mouse model. Materials and Methods: Apple pectin (4% wt/wt in drinking water) was administered to wild-type and pVillin-Cre-EGFP transgenic mice with intestinal fibrosis induced by a single dose of abdominal irradiation of 10 Gy. The effects of pectin on intestinal EMT and fibrosis, gut microbiota, and short-chain fatty acid (SCFA) concentration were evaluated. Results: Intestinal fibrosis in late radiation enteropathy showed increased submucosal thickness and subepithelial collagen deposition. Enhanced green fluorescent protein (EGFP)+/vimentin+ and EGFP+/α–smooth muscle actin (SMA)+ coexpressing cells were most clearly observed at 2 weeks after irradiation and gradually decreased at 4 and 12 weeks. Pectin significantly attenuated the thickness of submucosa and collagen deposition at 12 weeks (24.3 vs 27.6 µm in the pectin + radiation-treated group compared with radiation-alone group, respectively, P < .05; 69.0% vs 57.1%, P < .001) and ameliorated EMT at 2 and 4 weeks. Pectin also modulated the intestinal microbiota composition and increased the luminal SCFA concentration. Conclusion: The soluble dietary fiber pectin protected the terminal ileum against radiation-induced fibrosis. This effect might be mediated by altered SCFA concentration in the intestinal lumen and reduced EMT in the ileal epithelium.</description><identifier>ISSN: 0148-6071</identifier><identifier>EISSN: 1941-2444</identifier><identifier>DOI: 10.1177/0148607116671101</identifier><identifier>PMID: 27660288</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Dietary Fiber - pharmacology ; Disease Models, Animal ; Epithelial-Mesenchymal Transition - drug effects ; Epithelial-Mesenchymal Transition - radiation effects ; epithelial‐to‐mesenchymal transition ; Fatty Acids, Volatile - metabolism ; Fibrosis ; Gastrointestinal Microbiome - drug effects ; Ileum - drug effects ; Ileum - pathology ; Ileum - radiation effects ; intestinal fibrosis ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - pathology ; Intestinal Mucosa - radiation effects ; Intestines - drug effects ; Intestines - pathology ; Intestines - radiation effects ; Malus - chemistry ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Pectins - pharmacology ; radiation ; Radiotherapy - adverse effects ; soluble dietary fiber</subject><ispartof>JPEN. Journal of parenteral and enteral nutrition, 2017-11, Vol.41 (8), p.1399-1410</ispartof><rights>2016 American Society for Parenteral and Enteral Nutrition</rights><rights>2017 by The American Society for Parenteral and Enteral Nutrition</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3849-f23e091418a2c86bde363df7f1de4c997e6c99d32055c81438e1b11936e2fb6b3</citedby><cites>FETCH-LOGICAL-c3849-f23e091418a2c86bde363df7f1de4c997e6c99d32055c81438e1b11936e2fb6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1177%2F0148607116671101$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1177%2F0148607116671101$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27660288$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Jianbo</creatorcontrib><creatorcontrib>Ding, Chao</creatorcontrib><creatorcontrib>Dai, Xujie</creatorcontrib><creatorcontrib>Lv, Tengfei</creatorcontrib><creatorcontrib>Xie, Tingbing</creatorcontrib><creatorcontrib>Zhang, Tenghui</creatorcontrib><creatorcontrib>Gao, Wen</creatorcontrib><creatorcontrib>Gong, Jianfeng</creatorcontrib><creatorcontrib>Zhu, Weiming</creatorcontrib><creatorcontrib>Li, Ning</creatorcontrib><creatorcontrib>Li, Jieshou</creatorcontrib><title>Soluble Dietary Fiber Ameliorates Radiation-Induced Intestinal Epithelial-to-Mesenchymal Transition and Fibrosis</title><title>JPEN. Journal of parenteral and enteral nutrition</title><addtitle>JPEN J Parenter Enteral Nutr</addtitle><description>Background: Intestinal fibrosis is a late complication of pelvic radiotherapy. Epithelial-to-mesenchymal transition (EMT) plays an important role in tissue fibrosis. The aim of this study was to examine the effect of soluble dietary fiber on radiation-induced intestinal EMT and fibrosis in a mouse model. Materials and Methods: Apple pectin (4% wt/wt in drinking water) was administered to wild-type and pVillin-Cre-EGFP transgenic mice with intestinal fibrosis induced by a single dose of abdominal irradiation of 10 Gy. The effects of pectin on intestinal EMT and fibrosis, gut microbiota, and short-chain fatty acid (SCFA) concentration were evaluated. Results: Intestinal fibrosis in late radiation enteropathy showed increased submucosal thickness and subepithelial collagen deposition. Enhanced green fluorescent protein (EGFP)+/vimentin+ and EGFP+/α–smooth muscle actin (SMA)+ coexpressing cells were most clearly observed at 2 weeks after irradiation and gradually decreased at 4 and 12 weeks. Pectin significantly attenuated the thickness of submucosa and collagen deposition at 12 weeks (24.3 vs 27.6 µm in the pectin + radiation-treated group compared with radiation-alone group, respectively, P < .05; 69.0% vs 57.1%, P < .001) and ameliorated EMT at 2 and 4 weeks. Pectin also modulated the intestinal microbiota composition and increased the luminal SCFA concentration. Conclusion: The soluble dietary fiber pectin protected the terminal ileum against radiation-induced fibrosis. This effect might be mediated by altered SCFA concentration in the intestinal lumen and reduced EMT in the ileal epithelium.</description><subject>Animals</subject><subject>Dietary Fiber - pharmacology</subject><subject>Disease Models, Animal</subject><subject>Epithelial-Mesenchymal Transition - drug effects</subject><subject>Epithelial-Mesenchymal Transition - radiation effects</subject><subject>epithelial‐to‐mesenchymal transition</subject><subject>Fatty Acids, Volatile - metabolism</subject><subject>Fibrosis</subject><subject>Gastrointestinal Microbiome - drug effects</subject><subject>Ileum - drug effects</subject><subject>Ileum - pathology</subject><subject>Ileum - radiation effects</subject><subject>intestinal fibrosis</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - pathology</subject><subject>Intestinal Mucosa - radiation effects</subject><subject>Intestines - drug effects</subject><subject>Intestines - pathology</subject><subject>Intestines - radiation effects</subject><subject>Malus - chemistry</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Pectins - pharmacology</subject><subject>radiation</subject><subject>Radiotherapy - adverse effects</subject><subject>soluble dietary fiber</subject><issn>0148-6071</issn><issn>1941-2444</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1P2zAYxq0JtJZu952mHLkY_MauYx8RK1BUBhpwjpz4zXDlJJ2daOp_P0dlOyChXV5Lfj706EfIF2BnAEVxzkAoyQoAKdNh8IHMQQuguRDiiMwnmU76jJzEuGWMccnYRzLLCylZrtSc7B57P1Yes28OBxP22ZWrMGQXLXrXBzNgzH4Y68zg-o6uOzvWaLN1l_4H1xmfrXZueEle4-nQ0zuM2NUv-zYpT8F00U25zHR26g19dPETOW6Mj_j59V2Q56vV0-UN3dxfry8vNrTmSmja5ByZBgHK5LWSlUUuuW2KBiyKWusCZbqW52y5rBUIrhAqAM0l5k0lK74gp4feXeh_jWlu2bpYo_emw36MJailLvJETSQrO1jrtDAGbMpdcG2CUQIrJ87lW84p8vW1faxatP8Cf8EmgzwYfjuP-_8WlrcPq-_AtU5BeghG8xPLbT-GhDm-v-QPsCSVLA</recordid><startdate>201711</startdate><enddate>201711</enddate><creator>Yang, Jianbo</creator><creator>Ding, Chao</creator><creator>Dai, Xujie</creator><creator>Lv, Tengfei</creator><creator>Xie, Tingbing</creator><creator>Zhang, Tenghui</creator><creator>Gao, Wen</creator><creator>Gong, Jianfeng</creator><creator>Zhu, Weiming</creator><creator>Li, Ning</creator><creator>Li, Jieshou</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201711</creationdate><title>Soluble Dietary Fiber Ameliorates Radiation-Induced Intestinal Epithelial-to-Mesenchymal Transition and Fibrosis</title><author>Yang, Jianbo ; Ding, Chao ; Dai, Xujie ; Lv, Tengfei ; Xie, Tingbing ; Zhang, Tenghui ; Gao, Wen ; Gong, Jianfeng ; Zhu, Weiming ; Li, Ning ; Li, Jieshou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3849-f23e091418a2c86bde363df7f1de4c997e6c99d32055c81438e1b11936e2fb6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Dietary Fiber - pharmacology</topic><topic>Disease Models, Animal</topic><topic>Epithelial-Mesenchymal Transition - drug effects</topic><topic>Epithelial-Mesenchymal Transition - radiation effects</topic><topic>epithelial‐to‐mesenchymal transition</topic><topic>Fatty Acids, Volatile - metabolism</topic><topic>Fibrosis</topic><topic>Gastrointestinal Microbiome - drug effects</topic><topic>Ileum - drug effects</topic><topic>Ileum - pathology</topic><topic>Ileum - radiation effects</topic><topic>intestinal fibrosis</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - pathology</topic><topic>Intestinal Mucosa - radiation effects</topic><topic>Intestines - drug effects</topic><topic>Intestines - pathology</topic><topic>Intestines - radiation effects</topic><topic>Malus - chemistry</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Pectins - pharmacology</topic><topic>radiation</topic><topic>Radiotherapy - adverse effects</topic><topic>soluble dietary fiber</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Jianbo</creatorcontrib><creatorcontrib>Ding, Chao</creatorcontrib><creatorcontrib>Dai, Xujie</creatorcontrib><creatorcontrib>Lv, Tengfei</creatorcontrib><creatorcontrib>Xie, Tingbing</creatorcontrib><creatorcontrib>Zhang, Tenghui</creatorcontrib><creatorcontrib>Gao, Wen</creatorcontrib><creatorcontrib>Gong, Jianfeng</creatorcontrib><creatorcontrib>Zhu, Weiming</creatorcontrib><creatorcontrib>Li, Ning</creatorcontrib><creatorcontrib>Li, Jieshou</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>JPEN. Journal of parenteral and enteral nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Jianbo</au><au>Ding, Chao</au><au>Dai, Xujie</au><au>Lv, Tengfei</au><au>Xie, Tingbing</au><au>Zhang, Tenghui</au><au>Gao, Wen</au><au>Gong, Jianfeng</au><au>Zhu, Weiming</au><au>Li, Ning</au><au>Li, Jieshou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Soluble Dietary Fiber Ameliorates Radiation-Induced Intestinal Epithelial-to-Mesenchymal Transition and Fibrosis</atitle><jtitle>JPEN. Journal of parenteral and enteral nutrition</jtitle><addtitle>JPEN J Parenter Enteral Nutr</addtitle><date>2017-11</date><risdate>2017</risdate><volume>41</volume><issue>8</issue><spage>1399</spage><epage>1410</epage><pages>1399-1410</pages><issn>0148-6071</issn><eissn>1941-2444</eissn><abstract>Background: Intestinal fibrosis is a late complication of pelvic radiotherapy. Epithelial-to-mesenchymal transition (EMT) plays an important role in tissue fibrosis. The aim of this study was to examine the effect of soluble dietary fiber on radiation-induced intestinal EMT and fibrosis in a mouse model. Materials and Methods: Apple pectin (4% wt/wt in drinking water) was administered to wild-type and pVillin-Cre-EGFP transgenic mice with intestinal fibrosis induced by a single dose of abdominal irradiation of 10 Gy. The effects of pectin on intestinal EMT and fibrosis, gut microbiota, and short-chain fatty acid (SCFA) concentration were evaluated. Results: Intestinal fibrosis in late radiation enteropathy showed increased submucosal thickness and subepithelial collagen deposition. Enhanced green fluorescent protein (EGFP)+/vimentin+ and EGFP+/α–smooth muscle actin (SMA)+ coexpressing cells were most clearly observed at 2 weeks after irradiation and gradually decreased at 4 and 12 weeks. Pectin significantly attenuated the thickness of submucosa and collagen deposition at 12 weeks (24.3 vs 27.6 µm in the pectin + radiation-treated group compared with radiation-alone group, respectively, P < .05; 69.0% vs 57.1%, P < .001) and ameliorated EMT at 2 and 4 weeks. Pectin also modulated the intestinal microbiota composition and increased the luminal SCFA concentration. Conclusion: The soluble dietary fiber pectin protected the terminal ileum against radiation-induced fibrosis. This effect might be mediated by altered SCFA concentration in the intestinal lumen and reduced EMT in the ileal epithelium.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>27660288</pmid><doi>10.1177/0148607116671101</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Dietary Fiber - pharmacology Disease Models, Animal Epithelial-Mesenchymal Transition - drug effects Epithelial-Mesenchymal Transition - radiation effects epithelial‐to‐mesenchymal transition Fatty Acids, Volatile - metabolism Fibrosis Gastrointestinal Microbiome - drug effects Ileum - drug effects Ileum - pathology Ileum - radiation effects intestinal fibrosis Intestinal Mucosa - drug effects Intestinal Mucosa - pathology Intestinal Mucosa - radiation effects Intestines - drug effects Intestines - pathology Intestines - radiation effects Malus - chemistry Mice Mice, Inbred C57BL Mice, Transgenic Pectins - pharmacology radiation Radiotherapy - adverse effects soluble dietary fiber |
| title | Soluble Dietary Fiber Ameliorates Radiation-Induced Intestinal Epithelial-to-Mesenchymal Transition and Fibrosis |
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