Comparison of PCR assays targeting the multi-copy targets B1 gene and 529 bp repetitive element for detection of Toxoplasma gondii in swine muscle

The comparison of the sensitivities of two molecular assays designed to target the multi-copy sequences of the Toxoplasma gondii genomic B1 region and 529 bp-RE respectively, in detecting T. gondii in swine muscle was assessed. Diaphragm pillars were obtained from 498 slaughtered pigs managed in int...

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Veröffentlicht in:	Food microbiology 2017-05, Vol.63, p.213-216
Hauptverfasser:	Veronesi, Fabrizia, Santoro, Azzurra, Milardi, Giovanni Luigi, Diaferia, Manuela, Branciari, Raffaella, Miraglia, Dino, Cioffi, Attilia, Gabrielli, Simona, Ranucci, David
Format:	Artikel
Sprache:	eng
Schlagworte:	529 bp-RE Animals B1 gene Food Microbiology Genome, Protozoan Interspersed Repetitive Sequences Italy Muscle tissue Muscles - parasitology Polymerase Chain Reaction - methods Repetitive Sequences, Nucleic Acid Sensitivity and Specificity Swine Swine Diseases - diagnosis Swine Diseases - parasitology Toxoplasma - genetics Toxoplasma - isolation & purification Toxoplasma gondii Toxoplasmosis, Animal - diagnosis Toxoplasmosis, Animal - parasitology
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Zusammenfassung:	The comparison of the sensitivities of two molecular assays designed to target the multi-copy sequences of the Toxoplasma gondii genomic B1 region and 529 bp-RE respectively, in detecting T. gondii in swine muscle was assessed. Diaphragm pillars were obtained from 498 slaughtered pigs managed in intensive farms in Central Italy. Genomic DNA was extracted from the tissues and T. gondii-B1 and 529 bp-RE sequences were amplified by specific PCR protocols. Toxoplasma gondii DNA was detected in 165 samples (33.13%). There was a good correlation (κ = 0.77) between the results obtained targeting the two different genetic markers, however the 529 bp RE-PCR assay overall detected a significantly higher (P
ISSN:	0740-0020 1095-9998
DOI:	10.1016/j.fm.2016.11.022