G-Quadruplex formation using fluorescent oligonucleotides as a detection method for discriminating AGG trinucleotide repeats

G-Quadruplex formation using fluorescent oligonucleotides as a detection method for discriminating AGG trinucleotide repeats

We have developed a simple and sensitive system for detecting AGG trinucleotide repeats through the formation of intermolecular G-quadruplexes using a fluorescent oligonucleotide. The fluorescence signal increased rapidly and dramatically by 44.7-fold with respect to the low background signal in the...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2016-01, Vol.52 (86), p.12757-12760
Hauptverfasser: Park, Yoojin, Kim, Ki Tae, Kim, Byeang Hyean
Format: Artikel
Sprache:eng
Schlagworte:
Deoxyribonucleic acid
Fluorescence
Formations
Oligonucleotides
Ribonucleic acids
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Zusammenfassung:We have developed a simple and sensitive system for detecting AGG trinucleotide repeats through the formation of intermolecular G-quadruplexes using a fluorescent oligonucleotide. The fluorescence signal increased rapidly and dramatically by 44.7-fold with respect to the low background signal in the presence of RNA agg repeats and by 35.0-fold in the presence of DNA AGG repeats.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc06566j