Enzymatic synthesis and reverse transcription of RNAs incorporating 2'-O-carbamoyl uridine triphosphate

Enzymatic synthesis and the reverse transcription of RNAs containing 2'-O-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2'-O-carbamoyl uridine triphosphate (U TP). U TP was incorporated correctly into long RNAs, and its fidelity during reve...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2016-01, Vol.52 (87), p.12889-12892
Hauptverfasser:	Masaki, Yoshiaki, Ito, Hyugo, Oda, Yuki, Yamazaki, Kazufumi, Tago, Nobuhiro, Ohno, Kentaro, Ishii, Nozomi, Tsunoda, Hirosuke, Kanamori, Takashi, Ohkubo, Akihiro, Sekine, Mitsuo, Seio, Kohji
Format:	Artikel
Sprache:	eng
Schlagworte:	Ribonucleic acids Synthesis
Online-Zugang:	Volltext
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