Enzymatic synthesis and reverse transcription of RNAs incorporating 2'-O-carbamoyl uridine triphosphate

Enzymatic synthesis and reverse transcription of RNAs incorporating 2'-O-carbamoyl uridine triphosphate

Enzymatic synthesis and the reverse transcription of RNAs containing 2'-O-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2'-O-carbamoyl uridine triphosphate (U TP). U TP was incorporated correctly into long RNAs, and its fidelity during reve...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2016-01, Vol.52 (87), p.12889-12892
Hauptverfasser: Masaki, Yoshiaki, Ito, Hyugo, Oda, Yuki, Yamazaki, Kazufumi, Tago, Nobuhiro, Ohno, Kentaro, Ishii, Nozomi, Tsunoda, Hirosuke, Kanamori, Takashi, Ohkubo, Akihiro, Sekine, Mitsuo, Seio, Kohji
Format: Artikel
Sprache:eng
Schlagworte:
Aptamers, Nucleotide - chemical synthesis
DNA-Directed RNA Polymerases - metabolism
Humans
Reverse Transcription
Ribonucleic acids
Synthesis
Uridine Triphosphate - chemistry
Viral Proteins - metabolism
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Zusammenfassung:Enzymatic synthesis and the reverse transcription of RNAs containing 2'-O-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2'-O-carbamoyl uridine triphosphate (U TP). U TP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc05796a