DNA Nanostructures Carrying Stoichiometrically Definable Antibodies

Targeted drug delivery is one of the key challenges in cancer nanomedicine. Stoichiometric and spatial control over the antibodies placement on the nanomedicine vehicle holds a pivotal role to overcome this key challenge. Here, a DNA tetrahedral is designed with available conjugation sites on its vertices, allowing to bind one, two, or three cetuximab antibodies per DNA nanostructure. This stoichiometrically definable cetuximab conjugated DNA nanostructure shows enhanced targeting on the breast cancer cells, which results with higher overall killing efficacy of the cancer cells. Previously antibodies mediated targeting nanomedicine cannot define the number of antibodies being conjugated per particle. Through singular covalent bonds strategically placed at the vertices of DNA tetrahedron, nanomedicine carrying one, two, or three antibodies is now possible. Defining antibody numbers per particle increases the consistency and overall efficacy of the nanomedicine and brings molecular control of nanomedicine to another level.