COMPARATIVE FLOW CYTOMETRIC ANALYSIS OF MESENCHYMAL STEM CELLS ISOLATED BY DIFFERENT METHODS FOR REGENERATIVE MEDICINE
The term stem cell is used to denote an unspecialized progenitor cell residing in niches in various organs and tissues from which it can be recruited to replenish specific tissue as a self-renewal cell source with application in cell-based therapies and tissue engineering. In our study, human mesenc...
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Veröffentlicht in: | Studia Universitatis "Vasile Goldiș". Seria ș̦tiințele vieții 2013-04, Vol.23 (2), p.107-107 |
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Sprache: | eng |
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Zusammenfassung: | The term stem cell is used to denote an unspecialized progenitor cell residing in niches in various organs and tissues from which it can be recruited to replenish specific tissue as a self-renewal cell source with application in cell-based therapies and tissue engineering. In our study, human mesenchymal stem cells (multipotent mesenchymal stromal stem cells) (hMSC), plastic-adherent and fibroblastic-like cells, have been obtained from multiple biological sources: umbilical cord blood (UCB), bone marrow aspirate (BM) and cancellous bone (CB) and by several existing methods in the literature, in order to compare the cells to be used by coculture with differentiated cells for regeneration of damaged tissues. The magnetic separation method for isolating hMSC led to obtaining a pure culture compared to enzymatic digestion with collagenase and the separation on density gradient (Ficoll) resulted in a pure culture much smaller than the other two methods used. Flow cytometric analysis showed that after 3 weeks of cell cultivation, cells were CD73 positive and CD34 negative, suggesting that their stem phenotype has been preserved. By light microscopy images no significant differences have been observed in colony formation between the different hMSC isolation methods. |
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ISSN: | 1584-2363 1842-7963 |