Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility
Cryopreservation of bull semen is a common biotechnology procedure in cattle breeding. However, when the ejaculate is obtained by electroejaculation, wide variation is observed in the sperm/seminal plasma (SP) ratio that can affect the freezability of semen in this species. The removal of SP may imp...
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creator | Campanholi, Suzane Peres Monteiro, Fabio Morato Ribeiro Dias, Erika Aline Mercadante, Maria Eugênia Zerlotti de Paz, Claudia Cristina Paro Dell'Aqua Junior, José Antonio Papa, Frederico Ozanam Dell'Aqua, Camila de Paula Freitas Vantini, Roberta Garcia, Joaquim Mansano |
description | Cryopreservation of bull semen is a common biotechnology procedure in cattle breeding. However, when the ejaculate is obtained by electroejaculation, wide variation is observed in the sperm/seminal plasma (SP) ratio that can affect the freezability of semen in this species. The removal of SP may improve the quality of frozen bull semen. The objective of this study was to evaluate the effect of SP removal from the ejaculate on the cryopreservation of semen from 38 Nellore bulls collected by electroejaculation. After collection, the ejaculate was divided into three aliquots: (1) control (N) diluted to a concentration of 60 × 106 spermatozoa/mL and frozen with SP; (2) centrifugation (C) at ×600g for 10 minutes and the pellet resuspended and frozen at the same concentration as N; and (3) filtration (F) through SpermFilter and sperm recovered and frozen at the same concentration as N. After thawing, sperm kinetics, plasma and acrosome membrane integrity, mitochondrial membrane potential, oxidative stress, and in vitro fertility were evaluated. Statistical analysis was performed using the SAS 9.2 package, and differences were considered significant when P |
doi_str_mv | 10.1016/j.theriogenology.2016.10.008 |
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However, when the ejaculate is obtained by electroejaculation, wide variation is observed in the sperm/seminal plasma (SP) ratio that can affect the freezability of semen in this species. The removal of SP may improve the quality of frozen bull semen. The objective of this study was to evaluate the effect of SP removal from the ejaculate on the cryopreservation of semen from 38 Nellore bulls collected by electroejaculation. After collection, the ejaculate was divided into three aliquots: (1) control (N) diluted to a concentration of 60 × 106 spermatozoa/mL and frozen with SP; (2) centrifugation (C) at ×600g for 10 minutes and the pellet resuspended and frozen at the same concentration as N; and (3) filtration (F) through SpermFilter and sperm recovered and frozen at the same concentration as N. After thawing, sperm kinetics, plasma and acrosome membrane integrity, mitochondrial membrane potential, oxidative stress, and in vitro fertility were evaluated. Statistical analysis was performed using the SAS 9.2 package, and differences were considered significant when P < 0.05. Higher average path velocity and straight-line velocity were observed in the groups submitted to SP removal compared to the control group (P < 0.01). In contrast, filtered samples exhibited higher beat cross frequency, straightness, and linearity compared to the other groups. Plasma membrane integrity was reduced when SP was removed, but lower oxidative stress was observed in groups C and F (34.91 ± 2.95% and 31.63 ± 2.95%, respectively) compared to group N (57.39 ± 2.95%). However, the percentage of hatched blastocysts was similar in the N and F groups (21.22 ± 1.05% and 24.00 ± 1.05%, respectively) and higher compared to group C (18.83 ± 1.05%). In conclusion, removal of SP by centrifugation for bull semen freezing reduced the rate of in vitro–produced embryos, whereas filtration of prefrozen semen was found to be an efficient alternative in terms of semen freezability and in vitro production of bovine embryos.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2016.10.008</identifier><identifier>PMID: 28043341</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Bull ; Cattle ; Centrifugation ; Cryopreservation ; Cryopreservation - methods ; Cryopreservation - veterinary ; Ejaculation ; Electric Stimulation ; Fertility ; Fertilization in Vitro - veterinary ; Filtration ; Male ; Semen ; Semen Analysis ; Semen Preservation - methods ; Seminal plasma</subject><ispartof>Theriogenology, 2017-02, Vol.89, p.114-121</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-92a929eb13cd0bfb43796bda0ef4c515ffd3d7c493841e6354fb5d3648900d7c3</citedby><cites>FETCH-LOGICAL-c440t-92a929eb13cd0bfb43796bda0ef4c515ffd3d7c493841e6354fb5d3648900d7c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0093691X16304976$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28043341$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Campanholi, Suzane Peres</creatorcontrib><creatorcontrib>Monteiro, Fabio Morato</creatorcontrib><creatorcontrib>Ribeiro Dias, Erika Aline</creatorcontrib><creatorcontrib>Mercadante, Maria Eugênia Zerlotti</creatorcontrib><creatorcontrib>de Paz, Claudia Cristina Paro</creatorcontrib><creatorcontrib>Dell'Aqua Junior, José Antonio</creatorcontrib><creatorcontrib>Papa, Frederico Ozanam</creatorcontrib><creatorcontrib>Dell'Aqua, Camila de Paula Freitas</creatorcontrib><creatorcontrib>Vantini, Roberta</creatorcontrib><creatorcontrib>Garcia, Joaquim Mansano</creatorcontrib><title>Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>Cryopreservation of bull semen is a common biotechnology procedure in cattle breeding. However, when the ejaculate is obtained by electroejaculation, wide variation is observed in the sperm/seminal plasma (SP) ratio that can affect the freezability of semen in this species. The removal of SP may improve the quality of frozen bull semen. The objective of this study was to evaluate the effect of SP removal from the ejaculate on the cryopreservation of semen from 38 Nellore bulls collected by electroejaculation. After collection, the ejaculate was divided into three aliquots: (1) control (N) diluted to a concentration of 60 × 106 spermatozoa/mL and frozen with SP; (2) centrifugation (C) at ×600g for 10 minutes and the pellet resuspended and frozen at the same concentration as N; and (3) filtration (F) through SpermFilter and sperm recovered and frozen at the same concentration as N. After thawing, sperm kinetics, plasma and acrosome membrane integrity, mitochondrial membrane potential, oxidative stress, and in vitro fertility were evaluated. Statistical analysis was performed using the SAS 9.2 package, and differences were considered significant when P < 0.05. Higher average path velocity and straight-line velocity were observed in the groups submitted to SP removal compared to the control group (P < 0.01). In contrast, filtered samples exhibited higher beat cross frequency, straightness, and linearity compared to the other groups. Plasma membrane integrity was reduced when SP was removed, but lower oxidative stress was observed in groups C and F (34.91 ± 2.95% and 31.63 ± 2.95%, respectively) compared to group N (57.39 ± 2.95%). However, the percentage of hatched blastocysts was similar in the N and F groups (21.22 ± 1.05% and 24.00 ± 1.05%, respectively) and higher compared to group C (18.83 ± 1.05%). In conclusion, removal of SP by centrifugation for bull semen freezing reduced the rate of in vitro–produced embryos, whereas filtration of prefrozen semen was found to be an efficient alternative in terms of semen freezability and in vitro production of bovine embryos.</description><subject>Animals</subject><subject>Bull</subject><subject>Cattle</subject><subject>Centrifugation</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>Ejaculation</subject><subject>Electric Stimulation</subject><subject>Fertility</subject><subject>Fertilization in Vitro - veterinary</subject><subject>Filtration</subject><subject>Male</subject><subject>Semen</subject><subject>Semen Analysis</subject><subject>Semen Preservation - methods</subject><subject>Seminal plasma</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9q3DAQxkVpaDZpX6Ho0EMv3kqW7LWglxKSNhDIpYHehP6MUi22tJFsg98ij9Bn6ZNVZreF3nISM9_vm2H0IfSBki0ltP20344_Ifn4CCH28XHZ1qVbpC0h3Su0od1OVKxm9DXaECJY1Qr64xxd5LwnhLC2pW_Qed0RzhinG_R87RyYEUeHMww-qB4fepUHhRMMcS6lBhcTYJOWeEiQIc1q9DGsDh1nH2A1Qqn1qEplsV4w9GVmirBXZupPeDhxT5Pq_bhgFSz24fev2RcSO0ijX_tv0ZlTfYZ3p_cSPdxcf7_6Vt3df729-nJXGc7JWIlaiVqApsxYop3mbCdabRUBx01DG-csszvDBes4hZY13OnGspZ3gpAisEv08Tj3kOLTBHmUg88G-l4FiFOWtGsa0jZcdAX9fERNijkncPKQ_KDSIimRayZyL__PRK6ZrGrJpNjfnzZNegD7z_w3hALcHAEo984ekszGQzBgfSr_KG30L9v0B-LQq8Y</recordid><startdate>201702</startdate><enddate>201702</enddate><creator>Campanholi, Suzane Peres</creator><creator>Monteiro, Fabio Morato</creator><creator>Ribeiro Dias, Erika Aline</creator><creator>Mercadante, Maria Eugênia Zerlotti</creator><creator>de Paz, Claudia Cristina Paro</creator><creator>Dell'Aqua Junior, José Antonio</creator><creator>Papa, Frederico Ozanam</creator><creator>Dell'Aqua, Camila de Paula Freitas</creator><creator>Vantini, Roberta</creator><creator>Garcia, Joaquim Mansano</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201702</creationdate><title>Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility</title><author>Campanholi, Suzane Peres ; Monteiro, Fabio Morato ; Ribeiro Dias, Erika Aline ; Mercadante, Maria Eugênia Zerlotti ; de Paz, Claudia Cristina Paro ; Dell'Aqua Junior, José Antonio ; Papa, Frederico Ozanam ; Dell'Aqua, Camila de Paula Freitas ; Vantini, Roberta ; Garcia, Joaquim Mansano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-92a929eb13cd0bfb43796bda0ef4c515ffd3d7c493841e6354fb5d3648900d7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Bull</topic><topic>Cattle</topic><topic>Centrifugation</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - veterinary</topic><topic>Ejaculation</topic><topic>Electric Stimulation</topic><topic>Fertility</topic><topic>Fertilization in Vitro - veterinary</topic><topic>Filtration</topic><topic>Male</topic><topic>Semen</topic><topic>Semen Analysis</topic><topic>Semen Preservation - methods</topic><topic>Seminal plasma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Campanholi, Suzane Peres</creatorcontrib><creatorcontrib>Monteiro, Fabio Morato</creatorcontrib><creatorcontrib>Ribeiro Dias, Erika Aline</creatorcontrib><creatorcontrib>Mercadante, Maria Eugênia Zerlotti</creatorcontrib><creatorcontrib>de Paz, Claudia Cristina Paro</creatorcontrib><creatorcontrib>Dell'Aqua Junior, José Antonio</creatorcontrib><creatorcontrib>Papa, Frederico Ozanam</creatorcontrib><creatorcontrib>Dell'Aqua, Camila de Paula Freitas</creatorcontrib><creatorcontrib>Vantini, Roberta</creatorcontrib><creatorcontrib>Garcia, Joaquim Mansano</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Campanholi, Suzane Peres</au><au>Monteiro, Fabio Morato</au><au>Ribeiro Dias, Erika Aline</au><au>Mercadante, Maria Eugênia Zerlotti</au><au>de Paz, Claudia Cristina Paro</au><au>Dell'Aqua Junior, José Antonio</au><au>Papa, Frederico Ozanam</au><au>Dell'Aqua, Camila de Paula Freitas</au><au>Vantini, Roberta</au><au>Garcia, Joaquim Mansano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2017-02</date><risdate>2017</risdate><volume>89</volume><spage>114</spage><epage>121</epage><pages>114-121</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>Cryopreservation of bull semen is a common biotechnology procedure in cattle breeding. However, when the ejaculate is obtained by electroejaculation, wide variation is observed in the sperm/seminal plasma (SP) ratio that can affect the freezability of semen in this species. The removal of SP may improve the quality of frozen bull semen. The objective of this study was to evaluate the effect of SP removal from the ejaculate on the cryopreservation of semen from 38 Nellore bulls collected by electroejaculation. After collection, the ejaculate was divided into three aliquots: (1) control (N) diluted to a concentration of 60 × 106 spermatozoa/mL and frozen with SP; (2) centrifugation (C) at ×600g for 10 minutes and the pellet resuspended and frozen at the same concentration as N; and (3) filtration (F) through SpermFilter and sperm recovered and frozen at the same concentration as N. After thawing, sperm kinetics, plasma and acrosome membrane integrity, mitochondrial membrane potential, oxidative stress, and in vitro fertility were evaluated. Statistical analysis was performed using the SAS 9.2 package, and differences were considered significant when P < 0.05. Higher average path velocity and straight-line velocity were observed in the groups submitted to SP removal compared to the control group (P < 0.01). In contrast, filtered samples exhibited higher beat cross frequency, straightness, and linearity compared to the other groups. Plasma membrane integrity was reduced when SP was removed, but lower oxidative stress was observed in groups C and F (34.91 ± 2.95% and 31.63 ± 2.95%, respectively) compared to group N (57.39 ± 2.95%). However, the percentage of hatched blastocysts was similar in the N and F groups (21.22 ± 1.05% and 24.00 ± 1.05%, respectively) and higher compared to group C (18.83 ± 1.05%). In conclusion, removal of SP by centrifugation for bull semen freezing reduced the rate of in vitro–produced embryos, whereas filtration of prefrozen semen was found to be an efficient alternative in terms of semen freezability and in vitro production of bovine embryos.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28043341</pmid><doi>10.1016/j.theriogenology.2016.10.008</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Bull Cattle Centrifugation Cryopreservation Cryopreservation - methods Cryopreservation - veterinary Ejaculation Electric Stimulation Fertility Fertilization in Vitro - veterinary Filtration Male Semen Semen Analysis Semen Preservation - methods Seminal plasma |
title | Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility |
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