Effects of Bioactive Compounds on Odontogenic Differentiation and Mineralization
Direct pulp capping involves the placement of dental materials directly onto vital pulp tissues after deep caries removal to stimulate the regeneration of reparative dentin. This physical barrier will serve as a “biological seal” between these materials and the pulp tissue. Although numerous direct...
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Veröffentlicht in: | Journal of dental research 2017-01, Vol.96 (1), p.107-115 |
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creator | Phung, S. Lee, C. Hong, C. Song, M. Yi, J.K. Stevenson, R.G. Kang, M.K. Shin, K.-H. Park, N.-H. Kim, R.H. |
description | Direct pulp capping involves the placement of dental materials directly onto vital pulp tissues after deep caries removal to stimulate the regeneration of reparative dentin. This physical barrier will serve as a “biological seal” between these materials and the pulp tissue. Although numerous direct pulp capping materials are available, the use of small bioactive compounds that can potently stimulate and expedite reparative dentin formation is still underexplored. Here, the authors compared and evaluated the pro-osteogenic and pro-odontogenic effects of 4 small bioactive compounds— phenamil (Phen), purmorphamine (Pur), genistein (Gen), and metformin (Met). The authors found that these compounds at noncytotoxic concentrations induced differentiation and mineralization of preosteoblastic MC3T3-E1 cells and preodontoblastic dental pulp stem cells (DPSCs) in a dose-dependent manner. Among them, Phen consistently and potently induced differentiation and mineralization in vitro. A single treatment with Phen was sufficient to enhance the mineralization potential of DPSCs in vitro. More importantly, Phen-treated DPSCs showed enhanced odontogenic differentiation and mineralization in vivo. Our study suggests that these small bioactive compounds merit further study for their potential clinical use as pulp capping materials. |
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This physical barrier will serve as a “biological seal” between these materials and the pulp tissue. Although numerous direct pulp capping materials are available, the use of small bioactive compounds that can potently stimulate and expedite reparative dentin formation is still underexplored. Here, the authors compared and evaluated the pro-osteogenic and pro-odontogenic effects of 4 small bioactive compounds— phenamil (Phen), purmorphamine (Pur), genistein (Gen), and metformin (Met). The authors found that these compounds at noncytotoxic concentrations induced differentiation and mineralization of preosteoblastic MC3T3-E1 cells and preodontoblastic dental pulp stem cells (DPSCs) in a dose-dependent manner. Among them, Phen consistently and potently induced differentiation and mineralization in vitro. A single treatment with Phen was sufficient to enhance the mineralization potential of DPSCs in vitro. More importantly, Phen-treated DPSCs showed enhanced odontogenic differentiation and mineralization in vivo. Our study suggests that these small bioactive compounds merit further study for their potential clinical use as pulp capping materials.</description><identifier>ISSN: 0022-0345</identifier><identifier>EISSN: 1544-0591</identifier><identifier>DOI: 10.1177/0022034516675152</identifier><identifier>PMID: 28033065</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Amiloride - analogs & derivatives ; Amiloride - pharmacology ; Animals ; Bioactive compounds ; Calcification, Physiologic - drug effects ; Clinical outcomes ; Dental caries ; Dental pulp ; Dental Pulp - cytology ; Dental Pulp - growth & development ; Dentin ; Dentistry ; Dose-Response Relationship, Drug ; Genistein ; Genistein - pharmacology ; Metformin ; Metformin - pharmacology ; Mice ; Mice, Nude ; Mineralization ; Morpholines - pharmacology ; Odontogenesis - drug effects ; Phosphatase ; Proteins ; Purines - pharmacology ; Stem Cell Transplantation - methods ; Stem cells ; Teeth</subject><ispartof>Journal of dental research, 2017-01, Vol.96 (1), p.107-115</ispartof><rights>International & American Associations for Dental Research 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-fc8f3c8d44098bf855a69914d5d0c55897d0d92a9a9f8b5faa559e0026c1eb673</citedby><cites>FETCH-LOGICAL-c365t-fc8f3c8d44098bf855a69914d5d0c55897d0d92a9a9f8b5faa559e0026c1eb673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0022034516675152$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0022034516675152$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,777,781,21800,27905,27906,43602,43603</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28033065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Phung, S.</creatorcontrib><creatorcontrib>Lee, C.</creatorcontrib><creatorcontrib>Hong, C.</creatorcontrib><creatorcontrib>Song, M.</creatorcontrib><creatorcontrib>Yi, J.K.</creatorcontrib><creatorcontrib>Stevenson, R.G.</creatorcontrib><creatorcontrib>Kang, M.K.</creatorcontrib><creatorcontrib>Shin, K.-H.</creatorcontrib><creatorcontrib>Park, N.-H.</creatorcontrib><creatorcontrib>Kim, R.H.</creatorcontrib><title>Effects of Bioactive Compounds on Odontogenic Differentiation and Mineralization</title><title>Journal of dental research</title><addtitle>J Dent Res</addtitle><description>Direct pulp capping involves the placement of dental materials directly onto vital pulp tissues after deep caries removal to stimulate the regeneration of reparative dentin. This physical barrier will serve as a “biological seal” between these materials and the pulp tissue. Although numerous direct pulp capping materials are available, the use of small bioactive compounds that can potently stimulate and expedite reparative dentin formation is still underexplored. Here, the authors compared and evaluated the pro-osteogenic and pro-odontogenic effects of 4 small bioactive compounds— phenamil (Phen), purmorphamine (Pur), genistein (Gen), and metformin (Met). The authors found that these compounds at noncytotoxic concentrations induced differentiation and mineralization of preosteoblastic MC3T3-E1 cells and preodontoblastic dental pulp stem cells (DPSCs) in a dose-dependent manner. Among them, Phen consistently and potently induced differentiation and mineralization in vitro. A single treatment with Phen was sufficient to enhance the mineralization potential of DPSCs in vitro. More importantly, Phen-treated DPSCs showed enhanced odontogenic differentiation and mineralization in vivo. Our study suggests that these small bioactive compounds merit further study for their potential clinical use as pulp capping materials.</description><subject>Amiloride - analogs & derivatives</subject><subject>Amiloride - pharmacology</subject><subject>Animals</subject><subject>Bioactive compounds</subject><subject>Calcification, Physiologic - drug effects</subject><subject>Clinical outcomes</subject><subject>Dental caries</subject><subject>Dental pulp</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - growth & development</subject><subject>Dentin</subject><subject>Dentistry</subject><subject>Dose-Response Relationship, Drug</subject><subject>Genistein</subject><subject>Genistein - pharmacology</subject><subject>Metformin</subject><subject>Metformin - pharmacology</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Mineralization</subject><subject>Morpholines - pharmacology</subject><subject>Odontogenesis - drug effects</subject><subject>Phosphatase</subject><subject>Proteins</subject><subject>Purines - pharmacology</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem cells</subject><subject>Teeth</subject><issn>0022-0345</issn><issn>1544-0591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1LAzEQxYMotlbvnmTBi5fVSXaTTY5a6wdU6kHPS5qPktImdbMr6F9vaqtCwdPAvN-8mXkInWK4xLiqrgAIgaKkmLGKYkr2UB_TssyBCryP-ms5X-s9dBTjHAALwotD1CMcigIY7aPnkbVGtTELNrtxQarWvZtsGJar0Hmd2j6b6ODbMDPeqezWJbwxvnWydUmTXmdPzptGLtznd-sYHVi5iOZkWwfo9W70MnzIx5P7x-H1OFcFo21uFbeF4rosQfCp5ZRKJgQuNdWgKOWi0qAFkUIKy6fUSkmpMOkhprCZsqoYoIuN76oJb52Jbb10UZnFQnoTulhjTkuWchEioec76Dx0jU_X1aQAYIRgBomCDaWaEGNjbL1q3FI2HzWGep12vZt2GjnbGnfTpdG_Az_xJiDfAFHOzN_Wfw2_ALcMhdQ</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Phung, S.</creator><creator>Lee, C.</creator><creator>Hong, C.</creator><creator>Song, M.</creator><creator>Yi, J.K.</creator><creator>Stevenson, R.G.</creator><creator>Kang, M.K.</creator><creator>Shin, K.-H.</creator><creator>Park, N.-H.</creator><creator>Kim, R.H.</creator><general>SAGE Publications</general><general>SAGE PUBLICATIONS, INC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>U9A</scope><scope>7X8</scope></search><sort><creationdate>201701</creationdate><title>Effects of Bioactive Compounds on Odontogenic Differentiation and Mineralization</title><author>Phung, S. ; Lee, C. ; Hong, C. ; Song, M. ; Yi, J.K. ; Stevenson, R.G. ; Kang, M.K. ; Shin, K.-H. ; Park, N.-H. ; Kim, R.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-fc8f3c8d44098bf855a69914d5d0c55897d0d92a9a9f8b5faa559e0026c1eb673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Amiloride - analogs & derivatives</topic><topic>Amiloride - pharmacology</topic><topic>Animals</topic><topic>Bioactive compounds</topic><topic>Calcification, Physiologic - drug effects</topic><topic>Clinical outcomes</topic><topic>Dental caries</topic><topic>Dental pulp</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - growth & development</topic><topic>Dentin</topic><topic>Dentistry</topic><topic>Dose-Response Relationship, Drug</topic><topic>Genistein</topic><topic>Genistein - pharmacology</topic><topic>Metformin</topic><topic>Metformin - pharmacology</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Mineralization</topic><topic>Morpholines - pharmacology</topic><topic>Odontogenesis - drug effects</topic><topic>Phosphatase</topic><topic>Proteins</topic><topic>Purines - pharmacology</topic><topic>Stem Cell Transplantation - methods</topic><topic>Stem cells</topic><topic>Teeth</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Phung, S.</creatorcontrib><creatorcontrib>Lee, C.</creatorcontrib><creatorcontrib>Hong, C.</creatorcontrib><creatorcontrib>Song, M.</creatorcontrib><creatorcontrib>Yi, J.K.</creatorcontrib><creatorcontrib>Stevenson, R.G.</creatorcontrib><creatorcontrib>Kang, M.K.</creatorcontrib><creatorcontrib>Shin, K.-H.</creatorcontrib><creatorcontrib>Park, N.-H.</creatorcontrib><creatorcontrib>Kim, R.H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phung, S.</au><au>Lee, C.</au><au>Hong, C.</au><au>Song, M.</au><au>Yi, J.K.</au><au>Stevenson, R.G.</au><au>Kang, M.K.</au><au>Shin, K.-H.</au><au>Park, N.-H.</au><au>Kim, R.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Bioactive Compounds on Odontogenic Differentiation and Mineralization</atitle><jtitle>Journal of dental research</jtitle><addtitle>J Dent Res</addtitle><date>2017-01</date><risdate>2017</risdate><volume>96</volume><issue>1</issue><spage>107</spage><epage>115</epage><pages>107-115</pages><issn>0022-0345</issn><eissn>1544-0591</eissn><abstract>Direct pulp capping involves the placement of dental materials directly onto vital pulp tissues after deep caries removal to stimulate the regeneration of reparative dentin. This physical barrier will serve as a “biological seal” between these materials and the pulp tissue. Although numerous direct pulp capping materials are available, the use of small bioactive compounds that can potently stimulate and expedite reparative dentin formation is still underexplored. Here, the authors compared and evaluated the pro-osteogenic and pro-odontogenic effects of 4 small bioactive compounds— phenamil (Phen), purmorphamine (Pur), genistein (Gen), and metformin (Met). The authors found that these compounds at noncytotoxic concentrations induced differentiation and mineralization of preosteoblastic MC3T3-E1 cells and preodontoblastic dental pulp stem cells (DPSCs) in a dose-dependent manner. Among them, Phen consistently and potently induced differentiation and mineralization in vitro. A single treatment with Phen was sufficient to enhance the mineralization potential of DPSCs in vitro. More importantly, Phen-treated DPSCs showed enhanced odontogenic differentiation and mineralization in vivo. Our study suggests that these small bioactive compounds merit further study for their potential clinical use as pulp capping materials.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>28033065</pmid><doi>10.1177/0022034516675152</doi><tpages>9</tpages></addata></record> |
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subjects | Amiloride - analogs & derivatives Amiloride - pharmacology Animals Bioactive compounds Calcification, Physiologic - drug effects Clinical outcomes Dental caries Dental pulp Dental Pulp - cytology Dental Pulp - growth & development Dentin Dentistry Dose-Response Relationship, Drug Genistein Genistein - pharmacology Metformin Metformin - pharmacology Mice Mice, Nude Mineralization Morpholines - pharmacology Odontogenesis - drug effects Phosphatase Proteins Purines - pharmacology Stem Cell Transplantation - methods Stem cells Teeth |
title | Effects of Bioactive Compounds on Odontogenic Differentiation and Mineralization |
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