Effects of Bioactive Compounds on Odontogenic Differentiation and Mineralization

Direct pulp capping involves the placement of dental materials directly onto vital pulp tissues after deep caries removal to stimulate the regeneration of reparative dentin. This physical barrier will serve as a “biological seal” between these materials and the pulp tissue. Although numerous direct...

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Veröffentlicht in:Journal of dental research 2017-01, Vol.96 (1), p.107-115
Hauptverfasser: Phung, S., Lee, C., Hong, C., Song, M., Yi, J.K., Stevenson, R.G., Kang, M.K., Shin, K.-H., Park, N.-H., Kim, R.H.
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container_end_page 115
container_issue 1
container_start_page 107
container_title Journal of dental research
container_volume 96
creator Phung, S.
Lee, C.
Hong, C.
Song, M.
Yi, J.K.
Stevenson, R.G.
Kang, M.K.
Shin, K.-H.
Park, N.-H.
Kim, R.H.
description Direct pulp capping involves the placement of dental materials directly onto vital pulp tissues after deep caries removal to stimulate the regeneration of reparative dentin. This physical barrier will serve as a “biological seal” between these materials and the pulp tissue. Although numerous direct pulp capping materials are available, the use of small bioactive compounds that can potently stimulate and expedite reparative dentin formation is still underexplored. Here, the authors compared and evaluated the pro-osteogenic and pro-odontogenic effects of 4 small bioactive compounds— phenamil (Phen), purmorphamine (Pur), genistein (Gen), and metformin (Met). The authors found that these compounds at noncytotoxic concentrations induced differentiation and mineralization of preosteoblastic MC3T3-E1 cells and preodontoblastic dental pulp stem cells (DPSCs) in a dose-dependent manner. Among them, Phen consistently and potently induced differentiation and mineralization in vitro. A single treatment with Phen was sufficient to enhance the mineralization potential of DPSCs in vitro. More importantly, Phen-treated DPSCs showed enhanced odontogenic differentiation and mineralization in vivo. Our study suggests that these small bioactive compounds merit further study for their potential clinical use as pulp capping materials.
doi_str_mv 10.1177/0022034516675152
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subjects Amiloride - analogs & derivatives
Amiloride - pharmacology
Animals
Bioactive compounds
Calcification, Physiologic - drug effects
Clinical outcomes
Dental caries
Dental pulp
Dental Pulp - cytology
Dental Pulp - growth & development
Dentin
Dentistry
Dose-Response Relationship, Drug
Genistein
Genistein - pharmacology
Metformin
Metformin - pharmacology
Mice
Mice, Nude
Mineralization
Morpholines - pharmacology
Odontogenesis - drug effects
Phosphatase
Proteins
Purines - pharmacology
Stem Cell Transplantation - methods
Stem cells
Teeth
title Effects of Bioactive Compounds on Odontogenic Differentiation and Mineralization
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