β‑Lactone Synthetase Found in the Olefin Biosynthesis Pathway

The first β-lactone synthetase enzyme is reported, creating an unexpected link between the biosynthesis of olefinic hydrocarbons and highly functionalized natural products. The enzyme OleC, involved in the microbial biosynthesis of long-chain olefinic hydrocarbons, reacts with syn- and anti-β-hydrox...

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Veröffentlicht in:	Biochemistry (Easton) 2017-01, Vol.56 (2), p.348-351
Hauptverfasser:	Christenson, James K., Richman, Jack E., Jensen, Matthew R., Neufeld, Jennifer Y., Wilmot, Carrie M., Wackett, Lawrence P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkenes - metabolism Amino Acid Sequence Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological Products - metabolism biosynthesis Coenzyme A Ligases - genetics Coenzyme A Ligases - metabolism enzymes Gene Expression Regulation, Bacterial hydrocarbons Hydroxy Acids Lactones - metabolism Micrococcus luteus - enzymology Micrococcus luteus - genetics Multigene Family Operon sequence analysis Sequence Homology, Amino Acid Stenotrophomonas maltophilia - enzymology Stenotrophomonas maltophilia - genetics Streptomyces - enzymology Streptomyces - genetics
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Beschreibung
Zusammenfassung:	The first β-lactone synthetase enzyme is reported, creating an unexpected link between the biosynthesis of olefinic hydrocarbons and highly functionalized natural products. The enzyme OleC, involved in the microbial biosynthesis of long-chain olefinic hydrocarbons, reacts with syn- and anti-β-hydroxy acid substrates to yield cis- and trans-β-lactones, respectively. Protein sequence comparisons reveal that enzymes homologous to OleC are encoded in natural product gene clusters that generate β-lactone rings, suggesting a common mechanism of biosynthesis.
ISSN:	0006-2960 1520-4995 1520-4995
DOI:	10.1021/acs.biochem.6b01199