Enhanced regeneration potential of mobilized dental pulp stem cells from immature teeth
Objectives We have previously demonstrated that dental pulp stem cells (DPSCs) isolated from mature teeth by granulocyte colony‐stimulating factor (G‐CSF)‐induced mobilization method can enhance angiogenesis/vasculogenesis and improve pulp regeneration when compared with colony‐derived DPSCs. Howeve...
Gespeichert in:
| Veröffentlicht in: | Oral diseases 2017-07, Vol.23 (5), p.620-628 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 628 |
|---|---|
| container_issue | 5 |
| container_start_page | 620 |
| container_title | Oral diseases |
| container_volume | 23 |
| creator | Nakayama, H Iohara, K Hayashi, Y Okuwa, Y Kurita, K Nakashima, M |
| description | Objectives
We have previously demonstrated that dental pulp stem cells (DPSCs) isolated from mature teeth by granulocyte colony‐stimulating factor (G‐CSF)‐induced mobilization method can enhance angiogenesis/vasculogenesis and improve pulp regeneration when compared with colony‐derived DPSCs. However, the efficacy of this method in immature teeth with root‐formative stage has never been investigated. Therefore, the aim of this study was to examine the stemness, biological characteristics, and regeneration potential in mobilized DPSCs compared with colony‐derived DPSCs from immature teeth.
Materials and methods
Mobilized DPSCs isolated from immature teeth were compared to colony‐derived DPSCs using methods including flow cytometry, migration assays, mRNA expression of angiogenic/neurotrophic factor, and induced differentiation assays. They were also compared in trophic effects of the secretome. Regeneration potential was further compared in an ectopic tooth transplantation model.
Results
Mobilized DPSCs had higher migration ability and expressed more angiogenic/neurotrophic factors than DPSCs. The mobilized DPSC secretome produced a higher stimulatory effect on migration, immunomodulation, anti‐apoptosis, endothelial differentiation, and neurite extension. In addition, vascularization and pulp regeneration potential were higher in mobilized DPSCs than in DPSCs.
Conclusions
G‐CSF‐induced mobilization method enhances regeneration potential of colony‐derived DPSCs from immature teeth. |
| doi_str_mv | 10.1111/odi.12619 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1852683086</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1907257530</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4199-6274a9f1177f0d9fd0cd46f3f9e7d461122e1893ce9034f72aabaa1e93322c793</originalsourceid><addsrcrecordid>eNp1kEtLxTAQhYMovhf-AQm40UWvmaRtmqX4BuFuFN2F3HaikbapSYvorzd61YXgbGY4fBzOHEL2gM0gzbFv3Ax4CWqFbELJIGMVL1bTLYo8K7h42CBbMT4zBlIJvk42uFRSlEpukvvz_sn0NTY04CP2GMzofE8HP2I_OtNSb2nnF65174lpkpi0YWoHGkfsaI1tG6kNvqOu68w4BaQj4vi0Q9asaSPufu9tcndxfnt6ld3ML69PT26yOgelspLL3CgLIKVljbINq5u8tMIqlOkA4ByhUqJGxURuJTdmYQygEoLzOn2zTQ6XvkPwLxPGUXcufqYyPfopaqgKXlaCVWVCD_6gz34KfUqnQTHJC1kIlqijJVUHH2NAq4fgOhPeNDD92bZObeuvthO7_-04LTpsfsmfehNwvAReXYtv_zvp-dn10vIDvgOIvg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1907257530</pqid></control><display><type>article</type><title>Enhanced regeneration potential of mobilized dental pulp stem cells from immature teeth</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><creator>Nakayama, H ; Iohara, K ; Hayashi, Y ; Okuwa, Y ; Kurita, K ; Nakashima, M</creator><creatorcontrib>Nakayama, H ; Iohara, K ; Hayashi, Y ; Okuwa, Y ; Kurita, K ; Nakashima, M</creatorcontrib><description>Objectives
We have previously demonstrated that dental pulp stem cells (DPSCs) isolated from mature teeth by granulocyte colony‐stimulating factor (G‐CSF)‐induced mobilization method can enhance angiogenesis/vasculogenesis and improve pulp regeneration when compared with colony‐derived DPSCs. However, the efficacy of this method in immature teeth with root‐formative stage has never been investigated. Therefore, the aim of this study was to examine the stemness, biological characteristics, and regeneration potential in mobilized DPSCs compared with colony‐derived DPSCs from immature teeth.
Materials and methods
Mobilized DPSCs isolated from immature teeth were compared to colony‐derived DPSCs using methods including flow cytometry, migration assays, mRNA expression of angiogenic/neurotrophic factor, and induced differentiation assays. They were also compared in trophic effects of the secretome. Regeneration potential was further compared in an ectopic tooth transplantation model.
Results
Mobilized DPSCs had higher migration ability and expressed more angiogenic/neurotrophic factors than DPSCs. The mobilized DPSC secretome produced a higher stimulatory effect on migration, immunomodulation, anti‐apoptosis, endothelial differentiation, and neurite extension. In addition, vascularization and pulp regeneration potential were higher in mobilized DPSCs than in DPSCs.
Conclusions
G‐CSF‐induced mobilization method enhances regeneration potential of colony‐derived DPSCs from immature teeth.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.12619</identifier><identifier>PMID: 27973697</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Adolescent ; Angiogenesis ; Animals ; Apoptosis ; Cell Differentiation - drug effects ; Cell Movement ; Cells, Cultured ; Cerebrospinal fluid ; Colonies ; Colony-stimulating factor ; Culture Media, Conditioned - pharmacology ; Dental pulp ; Dental Pulp - cytology ; Dental Pulp - physiology ; dental pulp stem cells ; Dentistry ; Endothelial Cells - drug effects ; Flow cytometry ; Gene expression ; Granulocyte colony-stimulating factor ; Granulocyte Colony-Stimulating Factor - pharmacology ; Humans ; immature tooth ; Immunomodulation ; Leukocytes (granulocytic) ; Mice ; migration ; Molar, Third ; Neovascularization, Physiologic ; Neurites - drug effects ; neuronal extension ; Neurotrophic factors ; pulp regeneration ; Regeneration ; Secretome ; Stem cells ; Stem Cells - drug effects ; Stem Cells - physiology ; Teeth ; Tooth Root - physiology ; Tooth Root - transplantation ; Transplantation ; Transplantation, Heterologous ; Vascularization</subject><ispartof>Oral diseases, 2017-07, Vol.23 (5), p.620-628</ispartof><rights>2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2017 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4199-6274a9f1177f0d9fd0cd46f3f9e7d461122e1893ce9034f72aabaa1e93322c793</citedby><cites>FETCH-LOGICAL-c4199-6274a9f1177f0d9fd0cd46f3f9e7d461122e1893ce9034f72aabaa1e93322c793</cites><orcidid>0000-0002-3900-5808</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.12619$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.12619$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27973697$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakayama, H</creatorcontrib><creatorcontrib>Iohara, K</creatorcontrib><creatorcontrib>Hayashi, Y</creatorcontrib><creatorcontrib>Okuwa, Y</creatorcontrib><creatorcontrib>Kurita, K</creatorcontrib><creatorcontrib>Nakashima, M</creatorcontrib><title>Enhanced regeneration potential of mobilized dental pulp stem cells from immature teeth</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objectives
We have previously demonstrated that dental pulp stem cells (DPSCs) isolated from mature teeth by granulocyte colony‐stimulating factor (G‐CSF)‐induced mobilization method can enhance angiogenesis/vasculogenesis and improve pulp regeneration when compared with colony‐derived DPSCs. However, the efficacy of this method in immature teeth with root‐formative stage has never been investigated. Therefore, the aim of this study was to examine the stemness, biological characteristics, and regeneration potential in mobilized DPSCs compared with colony‐derived DPSCs from immature teeth.
Materials and methods
Mobilized DPSCs isolated from immature teeth were compared to colony‐derived DPSCs using methods including flow cytometry, migration assays, mRNA expression of angiogenic/neurotrophic factor, and induced differentiation assays. They were also compared in trophic effects of the secretome. Regeneration potential was further compared in an ectopic tooth transplantation model.
Results
Mobilized DPSCs had higher migration ability and expressed more angiogenic/neurotrophic factors than DPSCs. The mobilized DPSC secretome produced a higher stimulatory effect on migration, immunomodulation, anti‐apoptosis, endothelial differentiation, and neurite extension. In addition, vascularization and pulp regeneration potential were higher in mobilized DPSCs than in DPSCs.
Conclusions
G‐CSF‐induced mobilization method enhances regeneration potential of colony‐derived DPSCs from immature teeth.</description><subject>Adolescent</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Movement</subject><subject>Cells, Cultured</subject><subject>Cerebrospinal fluid</subject><subject>Colonies</subject><subject>Colony-stimulating factor</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Dental pulp</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - physiology</subject><subject>dental pulp stem cells</subject><subject>Dentistry</subject><subject>Endothelial Cells - drug effects</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Granulocyte colony-stimulating factor</subject><subject>Granulocyte Colony-Stimulating Factor - pharmacology</subject><subject>Humans</subject><subject>immature tooth</subject><subject>Immunomodulation</subject><subject>Leukocytes (granulocytic)</subject><subject>Mice</subject><subject>migration</subject><subject>Molar, Third</subject><subject>Neovascularization, Physiologic</subject><subject>Neurites - drug effects</subject><subject>neuronal extension</subject><subject>Neurotrophic factors</subject><subject>pulp regeneration</subject><subject>Regeneration</subject><subject>Secretome</subject><subject>Stem cells</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - physiology</subject><subject>Teeth</subject><subject>Tooth Root - physiology</subject><subject>Tooth Root - transplantation</subject><subject>Transplantation</subject><subject>Transplantation, Heterologous</subject><subject>Vascularization</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtLxTAQhYMovhf-AQm40UWvmaRtmqX4BuFuFN2F3HaikbapSYvorzd61YXgbGY4fBzOHEL2gM0gzbFv3Ax4CWqFbELJIGMVL1bTLYo8K7h42CBbMT4zBlIJvk42uFRSlEpukvvz_sn0NTY04CP2GMzofE8HP2I_OtNSb2nnF65174lpkpi0YWoHGkfsaI1tG6kNvqOu68w4BaQj4vi0Q9asaSPufu9tcndxfnt6ld3ML69PT26yOgelspLL3CgLIKVljbINq5u8tMIqlOkA4ByhUqJGxURuJTdmYQygEoLzOn2zTQ6XvkPwLxPGUXcufqYyPfopaqgKXlaCVWVCD_6gz34KfUqnQTHJC1kIlqijJVUHH2NAq4fgOhPeNDD92bZObeuvthO7_-04LTpsfsmfehNwvAReXYtv_zvp-dn10vIDvgOIvg</recordid><startdate>201707</startdate><enddate>201707</enddate><creator>Nakayama, H</creator><creator>Iohara, K</creator><creator>Hayashi, Y</creator><creator>Okuwa, Y</creator><creator>Kurita, K</creator><creator>Nakashima, M</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3900-5808</orcidid></search><sort><creationdate>201707</creationdate><title>Enhanced regeneration potential of mobilized dental pulp stem cells from immature teeth</title><author>Nakayama, H ; Iohara, K ; Hayashi, Y ; Okuwa, Y ; Kurita, K ; Nakashima, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4199-6274a9f1177f0d9fd0cd46f3f9e7d461122e1893ce9034f72aabaa1e93322c793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adolescent</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Movement</topic><topic>Cells, Cultured</topic><topic>Cerebrospinal fluid</topic><topic>Colonies</topic><topic>Colony-stimulating factor</topic><topic>Culture Media, Conditioned - pharmacology</topic><topic>Dental pulp</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - physiology</topic><topic>dental pulp stem cells</topic><topic>Dentistry</topic><topic>Endothelial Cells - drug effects</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Granulocyte colony-stimulating factor</topic><topic>Granulocyte Colony-Stimulating Factor - pharmacology</topic><topic>Humans</topic><topic>immature tooth</topic><topic>Immunomodulation</topic><topic>Leukocytes (granulocytic)</topic><topic>Mice</topic><topic>migration</topic><topic>Molar, Third</topic><topic>Neovascularization, Physiologic</topic><topic>Neurites - drug effects</topic><topic>neuronal extension</topic><topic>Neurotrophic factors</topic><topic>pulp regeneration</topic><topic>Regeneration</topic><topic>Secretome</topic><topic>Stem cells</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - physiology</topic><topic>Teeth</topic><topic>Tooth Root - physiology</topic><topic>Tooth Root - transplantation</topic><topic>Transplantation</topic><topic>Transplantation, Heterologous</topic><topic>Vascularization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakayama, H</creatorcontrib><creatorcontrib>Iohara, K</creatorcontrib><creatorcontrib>Hayashi, Y</creatorcontrib><creatorcontrib>Okuwa, Y</creatorcontrib><creatorcontrib>Kurita, K</creatorcontrib><creatorcontrib>Nakashima, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakayama, H</au><au>Iohara, K</au><au>Hayashi, Y</au><au>Okuwa, Y</au><au>Kurita, K</au><au>Nakashima, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced regeneration potential of mobilized dental pulp stem cells from immature teeth</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2017-07</date><risdate>2017</risdate><volume>23</volume><issue>5</issue><spage>620</spage><epage>628</epage><pages>620-628</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><abstract>Objectives
We have previously demonstrated that dental pulp stem cells (DPSCs) isolated from mature teeth by granulocyte colony‐stimulating factor (G‐CSF)‐induced mobilization method can enhance angiogenesis/vasculogenesis and improve pulp regeneration when compared with colony‐derived DPSCs. However, the efficacy of this method in immature teeth with root‐formative stage has never been investigated. Therefore, the aim of this study was to examine the stemness, biological characteristics, and regeneration potential in mobilized DPSCs compared with colony‐derived DPSCs from immature teeth.
Materials and methods
Mobilized DPSCs isolated from immature teeth were compared to colony‐derived DPSCs using methods including flow cytometry, migration assays, mRNA expression of angiogenic/neurotrophic factor, and induced differentiation assays. They were also compared in trophic effects of the secretome. Regeneration potential was further compared in an ectopic tooth transplantation model.
Results
Mobilized DPSCs had higher migration ability and expressed more angiogenic/neurotrophic factors than DPSCs. The mobilized DPSC secretome produced a higher stimulatory effect on migration, immunomodulation, anti‐apoptosis, endothelial differentiation, and neurite extension. In addition, vascularization and pulp regeneration potential were higher in mobilized DPSCs than in DPSCs.
Conclusions
G‐CSF‐induced mobilization method enhances regeneration potential of colony‐derived DPSCs from immature teeth.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>27973697</pmid><doi>10.1111/odi.12619</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-3900-5808</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1354-523X |
| ispartof | Oral diseases, 2017-07, Vol.23 (5), p.620-628 |
| issn | 1354-523X 1601-0825 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1852683086 |
| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Adolescent Angiogenesis Animals Apoptosis Cell Differentiation - drug effects Cell Movement Cells, Cultured Cerebrospinal fluid Colonies Colony-stimulating factor Culture Media, Conditioned - pharmacology Dental pulp Dental Pulp - cytology Dental Pulp - physiology dental pulp stem cells Dentistry Endothelial Cells - drug effects Flow cytometry Gene expression Granulocyte colony-stimulating factor Granulocyte Colony-Stimulating Factor - pharmacology Humans immature tooth Immunomodulation Leukocytes (granulocytic) Mice migration Molar, Third Neovascularization, Physiologic Neurites - drug effects neuronal extension Neurotrophic factors pulp regeneration Regeneration Secretome Stem cells Stem Cells - drug effects Stem Cells - physiology Teeth Tooth Root - physiology Tooth Root - transplantation Transplantation Transplantation, Heterologous Vascularization |
| title | Enhanced regeneration potential of mobilized dental pulp stem cells from immature teeth |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T02%3A17%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enhanced%20regeneration%20potential%20of%20mobilized%20dental%20pulp%20stem%20cells%20from%20immature%20teeth&rft.jtitle=Oral%20diseases&rft.au=Nakayama,%20H&rft.date=2017-07&rft.volume=23&rft.issue=5&rft.spage=620&rft.epage=628&rft.pages=620-628&rft.issn=1354-523X&rft.eissn=1601-0825&rft_id=info:doi/10.1111/odi.12619&rft_dat=%3Cproquest_cross%3E1907257530%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1907257530&rft_id=info:pmid/27973697&rfr_iscdi=true |