Identification and quantification of myosin heavy chain isoforms in bovine and porcine longissimus muscles by LC-MS/MS analysis

Identification and quantification of myosin heavy chain isoforms in bovine and porcine longissimus muscles by LC-MS/MS analysis

To identify and quantify the myosin heavy chain (MHC) isoforms in porcine and bovine longissimus thoracis (LT) muscles, LC-MS/MS and label-free quantification were conducted. 2a, 2x, 2b and slow isoforms were identified in porcine LT muscle, whereas bovine LT muscle contained 2a, 2x and slow isoform...

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Veröffentlicht in:Meat science 2017-03, Vol.125, p.143-151
Hauptverfasser: Kim, Gap-Don, Jeong, Tae-Chul, Cho, Kye Man, Jeong, Jin-Yeon
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cattle
Chromatography, Liquid
Label-free quantification
LC-MS/MS
Muscle, Skeletal - chemistry
Myosin heavy chain
Myosin Heavy Chains - analysis
Protein Isoforms - analysis
Reproducibility of Results
Swine
Tandem Mass Spectrometry
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Beschreibung
Zusammenfassung:To identify and quantify the myosin heavy chain (MHC) isoforms in porcine and bovine longissimus thoracis (LT) muscles, LC-MS/MS and label-free quantification were conducted. 2a, 2x, 2b and slow isoforms were identified in porcine LT muscle, whereas bovine LT muscle contained 2a, 2x and slow isoforms. The highest peak intensity (44.32%) of MHC 2b was observed in porcine LT muscle, whereas the peak intensity of MHC 2a was the highest (71.15%) in bovine LT muscle (p
ISSN:0309-1740
1873-4138
DOI:10.1016/j.meatsci.2016.12.002