Oxime Based Selective Fluorescent Sensor for Arsenate Ion in a Greener Way with Bio-Imaging Application

Oxime Based Selective Fluorescent Sensor for Arsenate Ion in a Greener Way with Bio-Imaging Application

A newly designed oxime based probe, 2,4-dihydroxyacetophenone-oxime (DHAO), was found to recognize H2AsO4– selectively with ∼3 fold "turn-on" fluorescence enhancement and LOD of 29 μM, K = (2.10 ± 0.54) × 104 M–1 in purely aqueous medium. The structures of the DHAO and its adduct with H2As...

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Veröffentlicht in:Analytical Sciences 2016/12/10, Vol.32(12), pp.1295-1300
Hauptverfasser: DOLAI, Malay, ALAM, Rabiul, KATARKAR, Atul, CHAUDHURI, Keya, ALI, Mahammad
Format: Artikel
Sprache:eng
Schlagworte:
Analytical Chemistry
Arsenates - analysis
Chemistry
DFT calculations
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Hep G2 Cells
Humans
intracellular imaging
Optical Imaging - methods
Oxime-imine based turn-on fluorescent sensor
Oximes - chemistry
selective detection of arsenate ion
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Beschreibung
Zusammenfassung:A newly designed oxime based probe, 2,4-dihydroxyacetophenone-oxime (DHAO), was found to recognize H2AsO4– selectively with ∼3 fold "turn-on" fluorescence enhancement and LOD of 29 μM, K = (2.10 ± 0.54) × 104 M–1 in purely aqueous medium. The structures of the DHAO and its adduct with H2AsO4– were optimized by DFT calculations. Intracellular imaging of As(V) in HepG2 cells demonstrate the possibilities of in vitro/in vivo applications for selective monitoring of such species.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.32.1295