Performance of a Real-time PCR–based Approach and Droplet Digital PCR in Detecting Human Parechovirus Type 3 RNA

Highlights • Real-time RT-PCR detection limits for HPeV3 have not been fully evaluated. • RT-ddPCR is the novel technique with precise quantitation of low-copy target genes. • Two-step RT-ddPCR was less variable and more specific than one-step RT-ddPCR. • Two-step RT-ddPCR detected HPeV3 RNA in real...

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Veröffentlicht in:	Journal of clinical virology 2016-11, Vol.84, p.27-31
Hauptverfasser:	Aizawa, Yuta, Koyama, Akihide, Ishihara, Tomohiko, Onodera, Osamu, Saitoh, Akihiko, M.D., Ph.D
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Cerebrospinal fluid Digital PCR Female Genotype Human parechovirus type 3 Humans Infant Infant, Newborn Infants Infectious Disease Limit of Detection Male Meningoencephalitis - diagnosis Meningoencephalitis - virology Neonates Parechovirus - genetics Parechovirus - isolation & purification Picornaviridae Infections - diagnosis Picornaviridae Infections - virology Real-time PCR Real-Time Polymerase Chain Reaction - methods RNA, Viral - blood RNA, Viral - cerebrospinal fluid RNA, Viral - genetics RNA, Viral - isolation & purification Sepsis - diagnosis Sepsis - virology Viral Load
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Zusammenfassung:	Highlights • Real-time RT-PCR detection limits for HPeV3 have not been fully evaluated. • RT-ddPCR is the novel technique with precise quantitation of low-copy target genes. • Two-step RT-ddPCR was less variable and more specific than one-step RT-ddPCR. • Two-step RT-ddPCR detected HPeV3 RNA in real-time RT-PCR-negative CSF samples.
ISSN:	1386-6532 1873-5967
DOI:	10.1016/j.jcv.2016.09.009