The EB66® cell line as a valuable cell substrate for MVA-based vaccines production

Highlights • EB66® cell line is a valuable cell substrate for production of MVA-based vaccine. • Pilot-scale production process was established that yields high titres. • Pilot-scale purification process has been developed. • Virus attenuation and transgene expression are maintained in EB66® cells....

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Veröffentlicht in:	Vaccine 2016-11, Vol.34 (48), p.5878-5885
Hauptverfasser:	Léon, Arnaud, David, Anne-Laure, Madeline, Brice, Guianvarc’h, Laurence, Dureau, Elodie, Champion-Arnaud, Patrick, Hebben, Matthias, Huss, Thierry, Chatrenet, Benoît, Schwamborn, Klaus
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Animals Antigens, Viral Aquatic birds Cell culture Cell Line Deoxyribonucleic acid DNA Ducks EB66® cells Embryo, Nonmammalian - cytology Fibroblasts Genetic engineering Genomes Glucose Good Manufacturing Practice Humans Industrialization Infections Manufacturing Manufacturing process Modified vaccinia virus Ankara Mutation Serial Passage Substrates Transgenes Vaccine production Vaccines Vaccines, Attenuated Vaccinia virus - genetics Vaccinia virus - immunology Vaccinia virus - pathogenicity Vaccinia virus - physiology Viral Vaccines Virus Cultivation Virus Replication Viruses
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Beschreibung
Zusammenfassung:	Highlights • EB66® cell line is a valuable cell substrate for production of MVA-based vaccine. • Pilot-scale production process was established that yields high titres. • Pilot-scale purification process has been developed. • Virus attenuation and transgene expression are maintained in EB66® cells.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2016.10.043