DNase concentration assay to obtain DNA-free RNA from sugarcane leaves

The success of gene expression studies, protein synthesis, and construction of cDNA libraries directly depends on the purity and integrity of the RNA used in these tests, as even minor amounts of contaminant DNA (

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Bibliographische Detailangaben
Veröffentlicht in:	Genetics and molecular research 2016-12, Vol.15 (4)
Hauptverfasser:	Santos, J A, Luz, G A, Oliveira, K P, Oliveira, L F, Andrade Júnior, A S, Valente, S E S, Lima, P S C
Format:	Artikel
Sprache:	eng
Schlagworte:	Deoxyribonucleases - metabolism DNA - metabolism Gene Expression Plant Leaves - genetics RNA, Plant - analysis RNA, Plant - standards Saccharum - genetics
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	The success of gene expression studies, protein synthesis, and construction of cDNA libraries directly depends on the purity and integrity of the RNA used in these tests, as even minor amounts of contaminant DNA (
ISSN:	1676-5680 1676-5680
DOI:	10.4238/gmr15049073