Modulation of pPS10 host range by DnaA

Summary Narrow‐host‐range plasmid pPS10, originally found in Pseudomonas savastanoi, is unable to replicate in other strains such as Escherichia coli. Here, we report that the establishment of pPS10 in E. coli can be achieved by a triple mutation in the dnaA gene of E. coli (dnaA403), leading to Q14...

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Veröffentlicht in:	Molecular microbiology 2002-10, Vol.46 (1), p.223-234
Hauptverfasser:	Maestro, Beatriz, Sanz, Jesús M., Faelen, Michel, Couturier, Martine, Díaz‐Orejas, Ramón, Fernández‐Tresguerres, Elena
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Chromosomes, Bacterial - genetics DNA Helicases DNA Replication DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Escherichia coli - genetics Genetic Complementation Test Mutation Plasmids - genetics Proteins - metabolism Replicon Sequence Analysis, DNA Trans-Activators
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