An efficient method for preparing high quality DNA from plant DNA libraries in lambda phage

An efficient method for preparing high quality DNA from plant DNA libraries in lambda phage

An efficient method has been developed to improve preparation of phage particles by ammonium sulfate precipitation and to yield high quality DNA. The method, that has been used to screen plant DNA libraries constructed in λvectors, is inexpensive, does not require purification of phage particles, an...

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Veröffentlicht in:Biotechnology letters 2002-07, Vol.24 (14), p.1199-1202
Hauptverfasser: MASSARDO, Domenica R, BORRELLI, Grazia M, DI FONZO, Natale, ALIFANO, Pietro, DEL GIUDICE, Luigi
Format: Artikel
Sprache:eng
Schlagworte:
Ammonium
Biological and medical sciences
Biotechnology
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbiology
Others
Various methods and equipments
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Zusammenfassung:An efficient method has been developed to improve preparation of phage particles by ammonium sulfate precipitation and to yield high quality DNA. The method, that has been used to screen plant DNA libraries constructed in λvectors, is inexpensive, does not require purification of phage particles, and can be used from either plate stocks or liquid lysates. Up to 1100 μg DNA was produced from 5 ml lysate obtained from agar plates.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1016111331733