A comparative study for lipase immobilization onto alginate based composite electrospun nanofibers with effective and enhanced stability

•We synthesized PEO/Alginate and PVA/Alginate nanofibers using electrospinning.•We immobilized lipase onto alginate based electrospun nanofibers successfully.•Results showed that nanofibers enhanced the stability properties of lipase. In this study, lipase was successfully immobilized on polyvinyl a...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:International journal of biological macromolecules 2017-03, Vol.96, p.302-311
Hauptverfasser: İspirli Doğaç, Yasemin, Deveci, İlyas, Mercimek, Bedrettin, Teke, Mustafa
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:•We synthesized PEO/Alginate and PVA/Alginate nanofibers using electrospinning.•We immobilized lipase onto alginate based electrospun nanofibers successfully.•Results showed that nanofibers enhanced the stability properties of lipase. In this study, lipase was successfully immobilized on polyvinyl alcohol/alginate and polyethylene oxide/alginate nanofibers that were prepared by electrospinning. Results showed that nanofibers (especially polyvinyl alcohol/alginate) enhanced the stability properties of lipase. When the free lipase lost its all activity after 40–60min at high temperatures, both lipase immobilized nanofibers kept almost 65–70% activity at the same time. The lipase immobilized poly vinyl alcohol/alginate and polyethylene oxide/alginate nanofibers protected approximately all of their activities until pH 9. Lipase immobilized polyvinyl alcohol/alginate and polyethylene oxide/alginate nanofibers maintained 60% of their activities after 14 and 7 reuses, respectively. The morphology of nanofibers was characterized by Scanning Electron Microscope, Fourier Transform Infrared Spectroscopy and Thermal Gravimetric Analyzer. As a result, this nanofiber production method, electrospinning, is simple, versatile and economical for preparing appropriate carrier to immobilize the enzymes.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2016.11.120