Development of a multiplex taqMan real-time PCR assay for typing of Mycoplasma pneumoniae based on type-specific indels identified through whole genome sequencing

Abstract We developed a multiplex real-time PCR assay for simultaneously detecting M. pneumoniae and typing into historically-defined P1 types. Typing was achieved based on the presence of short type-specific indels identified through whole genome sequencing. This assay was 100% specific compared to...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2017-03, Vol.87 (3), p.203-206
Hauptverfasser:	Wolff, Bernard J, Benitez, Alvaro J, Desai, Heta P, Morrison, Shatavia S, Diaz, Maureen H, Winchell, Jonas M
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence DNA, Bacterial - genetics Genome, Bacterial - genetics High-Throughput Nucleotide Sequencing - methods Humans Infectious Disease Internal Medicine M. pneumoniae Molecular Typing - methods Mycoplasma pneumoniae - classification Mycoplasma pneumoniae - genetics Next generation sequencing P1 typing Pneumonia, Mycoplasma - diagnosis Pneumonia, Mycoplasma - microbiology Real-time PCR Real-Time Polymerase Chain Reaction - methods Sensitivity and Specificity Sequence Alignment Sequence Analysis, DNA - methods
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Zusammenfassung:	Abstract We developed a multiplex real-time PCR assay for simultaneously detecting M. pneumoniae and typing into historically-defined P1 types. Typing was achieved based on the presence of short type-specific indels identified through whole genome sequencing. This assay was 100% specific compared to existing methods and may be useful during epidemiologic investigations.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2016.11.013