Size-Selective Harvesting of Extracellular Vesicles for Strategic Analyses Towards Tumor Diagnoses

Extracellular vesicles (EV), typified by exosomes or microvesicles, are expected to be effective diagnostic markers for cancers. The sizes of the vesicles range from 20 to 1000 nm, but the size-dependent variations of the contents of EVs are still poorly understood. We succeeded in the size-selectiv...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2017-06, Vol.182 (2), p.609-623
Hauptverfasser:	Manri, Chihiro, Yokoi, Takahide, Nishida, Hirokazu
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens Biochemistry Biotechnology Breast cancer Breast Neoplasms - diagnosis Breast Neoplasms - metabolism Cancer Cell adhesion Cell adhesion & migration Cell adhesion molecules Cell-Derived Microparticles - metabolism Chemistry Chemistry and Materials Science Colon Colon cancer Colorectal cancer Diagnostic systems Exosomes Exosomes - metabolism Extracellular vesicles Female Harvest Harvesting Humans MCF-7 Cells Medical diagnosis MicroRNAs - metabolism miRNA Molecular weight Neoplasm Proteins - metabolism Polyethylene glycol Polyethylene Glycols - chemistry Polyethylenes Reagents Ribonucleic acid RNA RNA, Neoplasm - metabolism Tumors Vesicles
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Zusammenfassung:	Extracellular vesicles (EV), typified by exosomes or microvesicles, are expected to be effective diagnostic markers for cancers. The sizes of the vesicles range from 20 to 1000 nm, but the size-dependent variations of the contents of EVs are still poorly understood. We succeeded in the size-selective harvesting of the vesicles by utilizing the molecular weight-dependent characteristics of a variety of polyethylene glycols (PEG) as precipitating reagents and analyzed the antigens displayed on the surfaces of the vesicles and the miRNAs included in the vesicles from each size group. As a result, the relatively larger (
ISSN:	0273-2289 1559-0291
DOI:	10.1007/s12010-016-2348-5