Dual-Stage Crosslinking of a Gel-Phase Bioink Improves Cell Viability and Homogeneity for 3D Bioprinting

Dual-Stage Crosslinking of a Gel-Phase Bioink Improves Cell Viability and Homogeneity for 3D Bioprinting

Current bioinks for cell‐based 3D bioprinting are not suitable for technology scale‐up due to the challenges of cell sedimentation, cell membrane damage, and cell dehydration. A novel bioink hydrogel is presented with dual‐stage crosslinking specifically designed to overcome these three major hurdle...

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Veröffentlicht in:Advanced healthcare materials 2016-10, Vol.5 (19), p.2488-2492
Hauptverfasser: Dubbin, Karen, Hori, Yuki, Lewis, Kazuomori K., Heilshorn, Sarah C.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bioengineering
bioink
biomaterials
bioprinting
Bioprinting - methods
Cell Line
Cell Survival - drug effects
Crosslinking
Health care
Homogeneity
Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology
Hydrogels
Mice
NIH 3T3 Cells
Patterning
Printing, Three-Dimensional
Sedimentation
Three dimensional printing
tissue engineering
Tissue Engineering - methods
Tissue Scaffolds
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Beschreibung
Zusammenfassung:Current bioinks for cell‐based 3D bioprinting are not suitable for technology scale‐up due to the challenges of cell sedimentation, cell membrane damage, and cell dehydration. A novel bioink hydrogel is presented with dual‐stage crosslinking specifically designed to overcome these three major hurdles. This bioink enables the direct patterning of highly viable, multicell type constructs with long‐term spatial fidelity.
ISSN:2192-2640
2192-2659
DOI:10.1002/adhm.201600636