Inhibitory effect of Sophora subprosrate polysaccharide on mitochondria oxidative stress induced by PCV-2 infection in RAW264.7 cells
In the present study, the inhibitory effect of Sophora subprosrate polysaccharide (SSP) on PCV-2-induced mitochondrial respiratory burst in RAW264.7 cells was first investigated. The findings suggested that SOD activity and the anti-superoxide anion radical activity of the RAW264.7 cells were signif...
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| Veröffentlicht in: | International journal of biological macromolecules 2017-02, Vol.95, p.608-617 |
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| creator | Su, Zi-Jie Yang, Jian Luo, Wen-Juan Wei, Ying-Yi Shuai, Xue-Hong Hu, Ting-Jun |
| description | In the present study, the inhibitory effect of Sophora subprosrate polysaccharide (SSP) on PCV-2-induced mitochondrial respiratory burst in RAW264.7 cells was first investigated. The findings suggested that SOD activity and the anti-superoxide anion radical activity of the RAW264.7 cells were significantly decreased after PCV-2 infection, and MnSOD mRNA levels were significantly decreased, while NOX2 mRNA levels and protein expression were increased. Meanwhile, the O2•− levels and mitochondrial membrane potentials were significantly increased. After treatment with SSP, significant increases in the activities of SOD, anti-superoxide anion radical activities, and MnSOD mRNA levels in the PCV-2 infected cells were observed. Meanwhile, significant increases in NOX2 mRNA levels and protein expression, O2•− levels and mitochondrial membrane potentials were also observed. The results showed that PCV2 infection resulted in the mitochondria oxidative stress of RAW264.7 cells as indicated by an increasing mitochondrial membrane potential, which was then inhibited by SSP. It was concluded that RAW264.7 cells treated with SSP could suffer from mitochondrial damage, which may be mediated by the inhibition of the mitochondrial membrane potential. |
| doi_str_mv | 10.1016/j.ijbiomac.2016.11.101 |
| format | Article |
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The findings suggested that SOD activity and the anti-superoxide anion radical activity of the RAW264.7 cells were significantly decreased after PCV-2 infection, and MnSOD mRNA levels were significantly decreased, while NOX2 mRNA levels and protein expression were increased. Meanwhile, the O2•− levels and mitochondrial membrane potentials were significantly increased. After treatment with SSP, significant increases in the activities of SOD, anti-superoxide anion radical activities, and MnSOD mRNA levels in the PCV-2 infected cells were observed. Meanwhile, significant increases in NOX2 mRNA levels and protein expression, O2•− levels and mitochondrial membrane potentials were also observed. The results showed that PCV2 infection resulted in the mitochondria oxidative stress of RAW264.7 cells as indicated by an increasing mitochondrial membrane potential, which was then inhibited by SSP. It was concluded that RAW264.7 cells treated with SSP could suffer from mitochondrial damage, which may be mediated by the inhibition of the mitochondrial membrane potential.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2016.11.101</identifier><identifier>PMID: 27908718</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Circovirus - physiology ; Gene Expression Regulation, Enzymologic - drug effects ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - metabolism ; Membrane Potential, Mitochondrial - drug effects ; Mice ; Mitochondria - drug effects ; Mitochondria - metabolism ; Mitochondria oxidative stress ; NADPH Oxidase 2 ; NADPH Oxidases - genetics ; NADPH Oxidases - metabolism ; Oxidative Stress - drug effects ; Porcine circovirus type 2 (PCV2) ; RAW 264.7 Cells ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sophora - chemistry ; Sophora subprosrate polysaccharide (SSP) ; Superoxide Dismutase - metabolism ; Superoxides - metabolism</subject><ispartof>International journal of biological macromolecules, 2017-02, Vol.95, p.608-617</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-e8ead98504be745ceb7dc243ba83ab1dc7d8377d46133d54572c7c2ce0c650d93</citedby><cites>FETCH-LOGICAL-c368t-e8ead98504be745ceb7dc243ba83ab1dc7d8377d46133d54572c7c2ce0c650d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2016.11.101$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27908718$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Zi-Jie</creatorcontrib><creatorcontrib>Yang, Jian</creatorcontrib><creatorcontrib>Luo, Wen-Juan</creatorcontrib><creatorcontrib>Wei, Ying-Yi</creatorcontrib><creatorcontrib>Shuai, Xue-Hong</creatorcontrib><creatorcontrib>Hu, Ting-Jun</creatorcontrib><title>Inhibitory effect of Sophora subprosrate polysaccharide on mitochondria oxidative stress induced by PCV-2 infection in RAW264.7 cells</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>In the present study, the inhibitory effect of Sophora subprosrate polysaccharide (SSP) on PCV-2-induced mitochondrial respiratory burst in RAW264.7 cells was first investigated. The findings suggested that SOD activity and the anti-superoxide anion radical activity of the RAW264.7 cells were significantly decreased after PCV-2 infection, and MnSOD mRNA levels were significantly decreased, while NOX2 mRNA levels and protein expression were increased. Meanwhile, the O2•− levels and mitochondrial membrane potentials were significantly increased. After treatment with SSP, significant increases in the activities of SOD, anti-superoxide anion radical activities, and MnSOD mRNA levels in the PCV-2 infected cells were observed. Meanwhile, significant increases in NOX2 mRNA levels and protein expression, O2•− levels and mitochondrial membrane potentials were also observed. The results showed that PCV2 infection resulted in the mitochondria oxidative stress of RAW264.7 cells as indicated by an increasing mitochondrial membrane potential, which was then inhibited by SSP. It was concluded that RAW264.7 cells treated with SSP could suffer from mitochondrial damage, which may be mediated by the inhibition of the mitochondrial membrane potential.</description><subject>Animals</subject><subject>Circovirus - physiology</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>Mice</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondria oxidative stress</subject><subject>NADPH Oxidase 2</subject><subject>NADPH Oxidases - genetics</subject><subject>NADPH Oxidases - metabolism</subject><subject>Oxidative Stress - drug effects</subject><subject>Porcine circovirus type 2 (PCV2)</subject><subject>RAW 264.7 Cells</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sophora - chemistry</subject><subject>Sophora subprosrate polysaccharide (SSP)</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Superoxides - metabolism</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctu2zAQJIoWjZP2FwIee5HCFUWRujUw-ggQoEWfR4KPFUzDEl1SCuoP6H-XgpNee1rsYGYfM4RcA6uBQXezr8PehjgaVzelrwFW_BnZgJJ9xRjjz8mGQQuVAs4uyGXO-4J2AtRLctHInikJakP-3E27YMMc04niMKCbaRzo13jcxWRoXuwxxZzMjPQYD6dsnNuZFDzSONGxyNwuTj4FQ-Pv4M0cHpDmOWHONEx-ceipPdHP2x9VU4B1fCjCMNEvtz-brq0ldXg45FfkxWAOGV8_1ivy_f27b9uP1f2nD3fb2_vK8U7NFSo0vleCtRZlKxxa6V3TcmsUNxa8k15xKX3bAedetEI2TrrGIXOdYL7nV-TNeW756teCedZjyOsFZsK4ZA2qFarphVip3ZnqigE54aCPKYwmnTQwvUag9_opAr1GoAFWvAivH3csdkT_T_bkeSG8PROwfPoQMOnsAk7Fq5CKQdrH8L8dfwGIupy5</recordid><startdate>201702</startdate><enddate>201702</enddate><creator>Su, Zi-Jie</creator><creator>Yang, Jian</creator><creator>Luo, Wen-Juan</creator><creator>Wei, Ying-Yi</creator><creator>Shuai, Xue-Hong</creator><creator>Hu, Ting-Jun</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201702</creationdate><title>Inhibitory effect of Sophora subprosrate polysaccharide on mitochondria oxidative stress induced by PCV-2 infection in RAW264.7 cells</title><author>Su, Zi-Jie ; Yang, Jian ; Luo, Wen-Juan ; Wei, Ying-Yi ; Shuai, Xue-Hong ; Hu, Ting-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-e8ead98504be745ceb7dc243ba83ab1dc7d8377d46133d54572c7c2ce0c650d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Circovirus - physiology</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Membrane Potential, Mitochondrial - drug effects</topic><topic>Mice</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondria oxidative stress</topic><topic>NADPH Oxidase 2</topic><topic>NADPH Oxidases - genetics</topic><topic>NADPH Oxidases - metabolism</topic><topic>Oxidative Stress - drug effects</topic><topic>Porcine circovirus type 2 (PCV2)</topic><topic>RAW 264.7 Cells</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sophora - chemistry</topic><topic>Sophora subprosrate polysaccharide (SSP)</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Superoxides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Su, Zi-Jie</creatorcontrib><creatorcontrib>Yang, Jian</creatorcontrib><creatorcontrib>Luo, Wen-Juan</creatorcontrib><creatorcontrib>Wei, Ying-Yi</creatorcontrib><creatorcontrib>Shuai, Xue-Hong</creatorcontrib><creatorcontrib>Hu, Ting-Jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Zi-Jie</au><au>Yang, Jian</au><au>Luo, Wen-Juan</au><au>Wei, Ying-Yi</au><au>Shuai, Xue-Hong</au><au>Hu, Ting-Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effect of Sophora subprosrate polysaccharide on mitochondria oxidative stress induced by PCV-2 infection in RAW264.7 cells</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2017-02</date><risdate>2017</risdate><volume>95</volume><spage>608</spage><epage>617</epage><pages>608-617</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>In the present study, the inhibitory effect of Sophora subprosrate polysaccharide (SSP) on PCV-2-induced mitochondrial respiratory burst in RAW264.7 cells was first investigated. The findings suggested that SOD activity and the anti-superoxide anion radical activity of the RAW264.7 cells were significantly decreased after PCV-2 infection, and MnSOD mRNA levels were significantly decreased, while NOX2 mRNA levels and protein expression were increased. Meanwhile, the O2•− levels and mitochondrial membrane potentials were significantly increased. After treatment with SSP, significant increases in the activities of SOD, anti-superoxide anion radical activities, and MnSOD mRNA levels in the PCV-2 infected cells were observed. Meanwhile, significant increases in NOX2 mRNA levels and protein expression, O2•− levels and mitochondrial membrane potentials were also observed. The results showed that PCV2 infection resulted in the mitochondria oxidative stress of RAW264.7 cells as indicated by an increasing mitochondrial membrane potential, which was then inhibited by SSP. 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| subjects | Animals Circovirus - physiology Gene Expression Regulation, Enzymologic - drug effects Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Membrane Potential, Mitochondrial - drug effects Mice Mitochondria - drug effects Mitochondria - metabolism Mitochondria oxidative stress NADPH Oxidase 2 NADPH Oxidases - genetics NADPH Oxidases - metabolism Oxidative Stress - drug effects Porcine circovirus type 2 (PCV2) RAW 264.7 Cells RNA, Messenger - genetics RNA, Messenger - metabolism Sophora - chemistry Sophora subprosrate polysaccharide (SSP) Superoxide Dismutase - metabolism Superoxides - metabolism |
| title | Inhibitory effect of Sophora subprosrate polysaccharide on mitochondria oxidative stress induced by PCV-2 infection in RAW264.7 cells |
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