Procoagulant role of microparticles in routine storage of packed red blood cells: potential risk for prothrombotic post-transfusion complications

During routine storage, packed red blood cells (PRBC) undergo biochemical and morphological changes including loss of red blood cell (RBC) membrane asymmetry and release of microparticles (MPs) bearing phosphatidylserine (PS), a procoagulant phospholipid. This study investigated the association betw...

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Veröffentlicht in:	Pathology 2017-01, Vol.49 (1), p.62-69
Hauptverfasser:	Aung, Htet Htet, Tung, John-Paul, Dean, Melinda M., Flower, Robert L., Pecheniuk, Natalie M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Blood coagulation Blood Coagulation - drug effects Blood Coagulation Tests - methods Blood Preservation - methods blood transfusion Cell-Derived Microparticles erythrocyte transfusion Erythrocytes - drug effects Flow Cytometry - methods Humans Phosphatidylserines - pharmacology phospholipids Risk Transfusion Reaction
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Zusammenfassung:	During routine storage, packed red blood cells (PRBC) undergo biochemical and morphological changes including loss of red blood cell (RBC) membrane asymmetry and release of microparticles (MPs) bearing phosphatidylserine (PS), a procoagulant phospholipid. This study investigated the association between PRBC storage duration, MP profile and procoagulant activity. Leukodepleted PRBC-supernatant (PRBC-SN; n=13) was prepared at weekly intervals throughout storage. Phospholipid-dependent procoagulant activity, assessed using a factor X-activated clotting time (XACT) assay, decreased throughout storage (p
ISSN:	0031-3025 1465-3931
DOI:	10.1016/j.pathol.2016.10.001