Postconditioning with sevoflurane ameliorates spatial learning and memory deficit after hemorrhage shock and resuscitation in rats
Abstract Background Severe hemorrhage shock and resuscitation is a systemic ischemia-reperfusion phenomenon which can induce learning and memory deficit in human and rats. Sevoflurane postconditioning has been proved to offer neuroprotection under different setting of cerebral ischemia –reperfusion...
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Veröffentlicht in: | The Journal of surgical research 2016-12, Vol.206 (2), p.307-315 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Abstract Background Severe hemorrhage shock and resuscitation is a systemic ischemia-reperfusion phenomenon which can induce learning and memory deficit in human and rats. Sevoflurane postconditioning has been proved to offer neuroprotection under different setting of cerebral ischemia –reperfusion in rats. The aim of this study was to investigate whether sevoflurane postconditioning could improve spatial learning and memory ability after hemorrhage shock and resuscitation in rats. Methods Thirty-five male rats were randomized into five groups: sham group, shock group, low concentration (sevo1, 1.2%), middle concentration (sevo2, 2.4%) and high concentration (sevo3, 3.6%) of sevoflurane postconditioning groups. The spatial learning and memory ability of rats was measured by Morris water maze (MWM) three days after the operation. The expression of choline acetyltransferase (CHAT) and acetylcholinesterase (ACHE) in the hippocampus CA1 region was observed by immunohistochemistry method after the MWM test. Results The ability of spatial learning and memory of rats and the expression of CHAT was significantly declined while the expression of ACHE increased in the shock group compared with the sham group ( P |
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ISSN: | 0022-4804 1095-8673 |
DOI: | 10.1016/j.jss.2016.08.026 |