Asymptomatic Infection With Mycoplasma hominis Negatively Affects Semen Parameters and Leads to Male Infertility as Confirmed by Improved Semen Parameters After Antibiotic Treatment

Objective To elucidate the association between asymptomatic infections caused by Mycoplasma hominis and male infertility and to evaluate the role of antibiotic therapy in the treatment of this failure. Materials and Methods A total of 165 infertile men having abnormal semen parameters (study group) as well as 165 healthy fertile men (control group) were included in this study. Semen samples were taken from all participants and, after analyzing for semen parameters, real-time polymerase chain reaction, microbial culture, and reactive oxygen species (ROS) as well as total antioxidant capacity (TAC) assays were performed. Infected individuals of the study group were treated with antibiotic. One month after the treatment completion, second semen samples were taken and all the tests mentioned were performed. The data were analyzed using SPSS statistical software, version 22.0. Results The frequency of M. hominis was significantly higher in the infertile men compared with the fertile ones (14.5% vs 3.6%, P = .001). The mean cycle threshold ( Ct ) value was lower in infected infertile men than in infected fertile men ( P