Cytotoxicity of the venom of Pelagia noctiluca forskål (Cnidaria: Scyphozoa)

The activity of Pelagia noctiluca venom was never assessed on cultured cells; therefore, we have evaluated on V79 cells the cytotoxicity, genotoxicity and ATP depletion induced after treatment. Venom did not cause alteration on cell DNA, but showed remarkable cytotoxic properties. With the highest n...

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Veröffentlicht in:	Toxicon (Oxford) 2002-06, Vol.40 (6), p.695-698
Hauptverfasser:	Mariottini, G.L, Sottofattori, E, Mazzei, M, Robbiano, L, Carli, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - analysis Animal poisons toxicology. Antivenoms Animals ATP Biological and medical sciences Cell Line Cell Survival - drug effects Chromatography, High Pressure Liquid Cnidaria Cnidaria - physiology Cnidarian Venoms - toxicity Cricetinae Cytotoxicity Dose-Response Relationship, Drug Fibroblasts - cytology Fibroblasts - drug effects Marine Medical sciences Pelagia noctiluca Scyphozoa Toxicology Venoms
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Zusammenfassung:	The activity of Pelagia noctiluca venom was never assessed on cultured cells; therefore, we have evaluated on V79 cells the cytotoxicity, genotoxicity and ATP depletion induced after treatment. Venom did not cause alteration on cell DNA, but showed remarkable cytotoxic properties. With the highest nematocyst concentration (150,000 nematocyst/ml) 74 and 39% cells survived after 1 and 3 h, respectively, when evaluated by Trypan blue. Treated cells showed increased ATP levels during the same time. Preliminary HPLC analyses have showed the occurrence of a protein containing peak.
ISSN:	0041-0101 1879-3150
DOI:	10.1016/S0041-0101(01)00262-8