Tapered-Tip Capillary Electrophoresis Nano-Electrospray Ionization Mass Spectrometry for Ultrasensitive Proteomics: the Mouse Cortex

Ultrasensitive characterization of the proteome raises the potential to understand how differential gene expression orchestrates cell heterogeneity in the brain. Here, we report a microanalytical capillary electrophoresis nano-flow electrospray ionization (CE-nanoESI) interface for mass spectrometry...

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Veröffentlicht in:	Journal of the American Society for Mass Spectrometry 2017-04, Vol.28 (4), p.597-607
Hauptverfasser:	Choi, Sam B., Zamarbide, Marta, Manzini, M. Chiara, Nemes, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Anchoring Bioinformatics Biotechnology Brain Capillary electrophoresis Capillary flow Chemistry Chemistry and Materials Science Dilution Electrophoresis Electrospraying Emitters Focus: Emerging Investigators: Research Article Gene expression Heterogeneity Ionization Ions Mass spectrometry Organic Chemistry Peptides Proteins Proteomics Quadrupoles Scientific imaging Spectroscopy Spraying
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Zusammenfassung:	Ultrasensitive characterization of the proteome raises the potential to understand how differential gene expression orchestrates cell heterogeneity in the brain. Here, we report a microanalytical capillary electrophoresis nano-flow electrospray ionization (CE-nanoESI) interface for mass spectrometry to enable the measurement of limited amounts of proteins in the mouse cortex. Our design integrates a custom-built CE system to a tapered-tip metal emitter in a co-axial sheath-flow configuration. This interface can be constructed in
ISSN:	1044-0305 1879-1123
DOI:	10.1007/s13361-016-1532-8