Saliva initiates the formation of pro-inflammatory macrophages in vitro

Highlights • Sterile saliva provoked a strong polarization into M1 macrophages indicated by IL-12 and IL-6 expression. • Arginase-1 and Ym1, both genes characteristic for the M2 phenotype, were not modulated by saliva. • Inhibition of TLR4 and NFκB signaling blocked saliva activity. • Autoclaving of...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Archives of oral biology 2017-01, Vol.73, p.295-301
Hauptverfasser:	Pourgonabadi, Solmaz, Müller, Heinz-Dieter, Mendes, Joao Rui, Gruber, Reinhard
Format:	Artikel
Sprache:	eng
Schlagworte:	Advanced Basic Science Animals Arginase - genetics Arginase - immunology Bone Marrow Cells - drug effects Bone Marrow Cells - metabolism Dentistry Inflammation Interleukin-12 - biosynthesis Interleukin-12 - genetics Interleukin-12 - immunology Interleukin-4 - genetics Interleukin-4 - immunology Interleukin-6 - biosynthesis Interleukin-6 - genetics Interleukin-6 - immunology Lipopolysaccharides - pharmacology Macrophage Activation - drug effects Macrophages - drug effects Macrophages - immunology Mice Mice, Inbred BALB C Mouse NF-kappa B - immunology NF-kappa B - metabolism Nitriles - pharmacology Phenotype Primary macrophages RAW 264.7 Cells RAW264.7 Saliva Saliva - immunology Saliva - metabolism Signal Transduction Sterilization Sulfonamides - pharmacology Sulfones - pharmacology TLR4 Toll-Like Receptor 4 - immunology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Highlights • Sterile saliva provoked a strong polarization into M1 macrophages indicated by IL-12 and IL-6 expression. • Arginase-1 and Ym1, both genes characteristic for the M2 phenotype, were not modulated by saliva. • Inhibition of TLR4 and NFκB signaling blocked saliva activity. • Autoclaving of saliva also reduced the polarization into M1 macrophages.
ISSN:	0003-9969 1879-1506
DOI:	10.1016/j.archoralbio.2016.10.012