Production of β-(pyrazol-1-yl)-L-alanine from L-serine and pyrazol using recombinant Escherichia coli cells expressing serine acetyltransferase and O-acetylserine sulfhydrylase-A

beta -(Pyrazol-1-yl)-L-alanine ( beta -PA) was produced from L-serine and pyrazol using recombinant Escherichia coli cells expressing serine acetyltransferase and O-acetylserine sulfhydrylase-A. The amount of beta -PA increased with increasing L-serine concentrations up to 600 mM at 50 mM pyrazol wh...

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Veröffentlicht in:Biotechnology letters 2001-12, Vol.23 (24), p.2051-2055
Hauptverfasser: MINO, Koshiki, YAMANOUE, Tsuyoshi, OHNO, Katsuhiro, SAKIYAMA, Takaharu, EISAKI, Naoki, MATSUYAMA, Asahi, NAKAMISHI, Kazuhiro
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Sprache:eng
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Zusammenfassung:beta -(Pyrazol-1-yl)-L-alanine ( beta -PA) was produced from L-serine and pyrazol using recombinant Escherichia coli cells expressing serine acetyltransferase and O-acetylserine sulfhydrylase-A. The amount of beta -PA increased with increasing L-serine concentrations up to 600 mM at 50 mM pyrazol while 100 mM pyrazol gave the highest beta -PA production with 50 mM L-serine. Under the optimized conditions, beta -PA accumulated in the broth at approximately 140 mM with a conversion of 90% with respect to the added amount of pyrazol.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1013799915951