Active paraplegics are protected against exercise-induced oxidative damage through the induction of antioxidant enzymes

Study design: Experimental study. Objectives: Exercise improves functional capacity in spinal cord injury (SCI). However, exhaustive exercise, especially when sporadic, is linked to the production of reactive oxygen species that may have a detrimental effect on SCI. We aimed to study the effect of a single bout of exhaustive exercise on systemic oxidative stress parameters and on the expression of antioxidant enzymes in individuals with paraplegia. Setting: The study was conducted in the Physical Therapy department and the Physical Education and Sports department of the University of Valencia. Methods: Sixteen paraplegic subjects were submitted to a graded exercise test (GET) until volitional exhaustion. They were divided into active or non-active groups. Blood samples were drawn immediately, 1 and 2 h after the GET. We determined plasma malondialdehyde (MDA) and protein carbonylation as markers of oxidative damage. Antioxidant gene expression (catalase and glutathione peroxidase–GPx) was determined in peripheral blood mononuclear cells. Results: We found a significant increase in plasma MDA and protein carbonyls immediately after the GET ( P