Omega 3 fatty acids (linolenic acid) in shellfish totok Polymesoda Erosa fed Tetraselmis chuii and Skeletonema costatum

The aim of the present study is to investigate me effect of natural food i.e. Tetraselmis chuii dan Skeletonema costatum on the content of omega-3 fatty acid (linolenic fatty acid) in marsh clam Polymesoda erosa. The clams used in this experiment were collection from Gombol waters. Segara Anakan, Cilacap with 5-6 cm in shell length Laboratory experimental method was used In the present research completely randomized design (RAL) with two factors and three replication was used in the experiment. The first factor was natural food (A) with three treatments i.e. T. chuii with concentration is 45 x 10 super(4) sel/ml (A1); S. costa tum with concentration Is 45 x 10 super(4) sel/ml (A2); and the combination of both i.e. 22.5 x 10 super(4) sel/ml T. chuii : 22.5 x 10 super(4) sel/ml S. costatum (A3). The second factor was four levels of sampling period time i.e. 2, 4, 6, and 8 days. Gas Liquid Chromatography method was used to content of omega-3 fatty acid (linolenic fatty add). All the data were analyzed by using Anova test with SPSS software. The result shows that, natural foods slgnificanly influence me content of omega-3 fatty acid (linolenic fatty acid) (F test= 5.409; p= 0.012). The sampling period time didn't influence the content of linolenic add to Marsh Clam P. erosa (F test= 0.795: p= 0.509). There is interaction effect between of natural food and sampling period significant influence the content of linolenic acid to Marsh Clam P. erosa (F test= 3.535; p= 0.012). The results suggest that the food quality, character, and to content cell wall two natural food influence the content of linolenic fatty acid to Marsh Clam P. erosa.Original Abstract: Percobaan ini dilakukan untuk mendapatkan informasi tentang pengaruh pemberian pakan alami Tetraselmis chuii dan Skeletonema costatum terhadap kandungan asam lemak omega-3 (asam Linolenat) pada kerang Totok Polymesoda erosa. Kerang Totok yang digunakan berukuran 5-6 cm berasal dari perairan P. Gombol, Segara Anakan Cilacap. Metode yang digunakan adalah eksperimental laboratorium dengan Rancangan Acak Lengkap (RAL) dengan 2 faktor dan 3 ulangan. Faktor pertama adalah pakan alami (A) dengan 3 perlakuan yaitu T chuii dengan konsentrasi 45x10 super(4) sel/ml (A1); S. costatum dengan konsentrasi 45 x 10 super(4) sel/ml (A2); dan kombinasi dari kedua pakan tersebut dengan konsentrasi 22,5 x 10 super(4) sel/ml T. chuii: 22,5 x 10 super(4) sel/ml S. costatum (A3). Faktor kedua yaitu periode waktu sampling (T) terdiri dar