Abstract 1435: Psychoimmunological effect of depression on cervical carcinoma: Genome-wide long noncoding RNA implications

Objective: Cervical cancer is the second most commonly diagnosed cancer and the third leading cause of cancer death among females in developing countries. Depression is a vital psychological factor for tumor progression. Long noncoding RNAs(LncRNAs) are a novel group of gene regulators and may contribute to the disease development. Herein, the study aims to discover the mechanisms of psychoimmunological effect of depression on cervical carcinoma from the perspective of long noncoding ribonucleic acid. Methods: Six tumor tissues were obtained from Ib-IIa invasive squamous cell cervical carcinoma patients during laproscopic surgeries. They were divided into high-depressed group and low-depressed group according to the scoring system. LncRNA microarray experiments were performed. Expression levels of six lncRNA-mRNA pairs were confirmed by quantitative real-time PCR in other 20 patients’ materials. Results: We found that 1621 lncRNA transcripts are differently expressed between high-depressed and paired low-depressed samples, with 510 up-regulated and 1111 down-regulated (fold-change>2.0). Most of lncRNAs were found trans-regulated by three TFs with most credentiality: BCL11A,MEF2A and TBP. In the KEGG analysis, the most down-regulated mRNA involved was systemic lupus erythematosus (Maximun transcriptional domain coverage: 25.5%). In the LncRNA and mRNA Coexpression, the function of lncRNAs with most differently expressions was predicted in relationship with interleukin-1 receptor activity (Number of lncRNAs annotated: 15). Conclusions: Our study identified that genome-wide LncRNA profiles are significantly altered in cervical carcinoma patients with worse depressed profiles, and the psychoimmunological effect of tumor progression needs further investigation. Keywords: Depression, Cervical Cancer, Immunology, Long-noncoding RNA Citation Format: Wanjun Dai, Yinhua Yu, Yu Kang. Psychoimmunological effect of depression on cervical carcinoma: Genome-wide long noncoding RNA implications. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1435.