Sol–Gel-Based Titania–Silica Thin Film Overlay for Long Period Fiber Grating-Based Biosensors
An evanescent wave optical fiber biosensor based on titania–silica-coated long period grating (LPG) is presented. The chemical overlay, which increases the refractive index (RI) sensitivity of the sensor, consists of a sol–gel-based titania–silica thin film, deposited along the sensing portion of th...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2015-12, Vol.87 (24), p.12024-12031 |
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| creator | Chiavaioli, Francesco Biswas, Palas Trono, Cosimo Jana, Sunirmal Bandyopadhyay, Somnath Basumallick, Nandini Giannetti, Ambra Tombelli, Sara Bera, Susanta Mallick, Aparajita Baldini, Francesco |
| description | An evanescent wave optical fiber biosensor based on titania–silica-coated long period grating (LPG) is presented. The chemical overlay, which increases the refractive index (RI) sensitivity of the sensor, consists of a sol–gel-based titania–silica thin film, deposited along the sensing portion of the fiber by means of the dip-coating technique. Changing both the sol viscosity and the withdrawal speed during the dip-coating made it possible to adjust the thickness of the film overlay, which is a crucial parameter for the sensor performance. After the functionalization of the fiber surface using a methacrylic acid/methacrylate copolymer, an antibody/antigen (IgG/anti-IgG) assay was carried out to assess the performance of sol–gel based titania–silica-coated LPGs as biosensors. The analyte concentration was determined from the wavelength shift at the end of the binding process and from the initial binding rate. This is the first time that a sol–gel based titania–silica-coated LPG is proposed as an effective and feasible label-free biosensor. The specificity of the sensor was validated by performing the same model assay after spiking anti-IgG into human serum. With this structured LPG, detection limits of the order of tens of micrograms per liter (10–11 M) are attained. |
| doi_str_mv | 10.1021/acs.analchem.5b01841 |
| format | Article |
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The chemical overlay, which increases the refractive index (RI) sensitivity of the sensor, consists of a sol–gel-based titania–silica thin film, deposited along the sensing portion of the fiber by means of the dip-coating technique. Changing both the sol viscosity and the withdrawal speed during the dip-coating made it possible to adjust the thickness of the film overlay, which is a crucial parameter for the sensor performance. After the functionalization of the fiber surface using a methacrylic acid/methacrylate copolymer, an antibody/antigen (IgG/anti-IgG) assay was carried out to assess the performance of sol–gel based titania–silica-coated LPGs as biosensors. The analyte concentration was determined from the wavelength shift at the end of the binding process and from the initial binding rate. This is the first time that a sol–gel based titania–silica-coated LPG is proposed as an effective and feasible label-free biosensor. The specificity of the sensor was validated by performing the same model assay after spiking anti-IgG into human serum. With this structured LPG, detection limits of the order of tens of micrograms per liter (10–11 M) are attained.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.5b01841</identifier><identifier>PMID: 26548589</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Binding ; Biological Assay ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biosensors ; Copolymers ; Dip coatings ; Fibers ; Humans ; Immunoglobulins - blood ; LPG ; Reproducibility of Results ; Sensitivity and Specificity ; Sensors ; Silica ; Silica Gel - chemistry ; Sol gel process ; Surface Properties ; Thin films ; Titanium - chemistry ; Viscosity</subject><ispartof>Analytical chemistry (Washington), 2015-12, Vol.87 (24), p.12024-12031</ispartof><rights>Copyright © 2015 American Chemical Society</rights><rights>Copyright American Chemical Society Dec 15, 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a508t-8c1b3217e3261eee60d65ca64db2a3dbead286ed602bf524ca6bde1e068673663</citedby><cites>FETCH-LOGICAL-a508t-8c1b3217e3261eee60d65ca64db2a3dbead286ed602bf524ca6bde1e068673663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.5b01841$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.5b01841$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26548589$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chiavaioli, Francesco</creatorcontrib><creatorcontrib>Biswas, Palas</creatorcontrib><creatorcontrib>Trono, Cosimo</creatorcontrib><creatorcontrib>Jana, Sunirmal</creatorcontrib><creatorcontrib>Bandyopadhyay, Somnath</creatorcontrib><creatorcontrib>Basumallick, Nandini</creatorcontrib><creatorcontrib>Giannetti, Ambra</creatorcontrib><creatorcontrib>Tombelli, Sara</creatorcontrib><creatorcontrib>Bera, Susanta</creatorcontrib><creatorcontrib>Mallick, Aparajita</creatorcontrib><creatorcontrib>Baldini, Francesco</creatorcontrib><title>Sol–Gel-Based Titania–Silica Thin Film Overlay for Long Period Fiber Grating-Based Biosensors</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>An evanescent wave optical fiber biosensor based on titania–silica-coated long period grating (LPG) is presented. The chemical overlay, which increases the refractive index (RI) sensitivity of the sensor, consists of a sol–gel-based titania–silica thin film, deposited along the sensing portion of the fiber by means of the dip-coating technique. Changing both the sol viscosity and the withdrawal speed during the dip-coating made it possible to adjust the thickness of the film overlay, which is a crucial parameter for the sensor performance. After the functionalization of the fiber surface using a methacrylic acid/methacrylate copolymer, an antibody/antigen (IgG/anti-IgG) assay was carried out to assess the performance of sol–gel based titania–silica-coated LPGs as biosensors. The analyte concentration was determined from the wavelength shift at the end of the binding process and from the initial binding rate. This is the first time that a sol–gel based titania–silica-coated LPG is proposed as an effective and feasible label-free biosensor. The specificity of the sensor was validated by performing the same model assay after spiking anti-IgG into human serum. 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Chem</addtitle><date>2015-12-15</date><risdate>2015</risdate><volume>87</volume><issue>24</issue><spage>12024</spage><epage>12031</epage><pages>12024-12031</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>An evanescent wave optical fiber biosensor based on titania–silica-coated long period grating (LPG) is presented. The chemical overlay, which increases the refractive index (RI) sensitivity of the sensor, consists of a sol–gel-based titania–silica thin film, deposited along the sensing portion of the fiber by means of the dip-coating technique. Changing both the sol viscosity and the withdrawal speed during the dip-coating made it possible to adjust the thickness of the film overlay, which is a crucial parameter for the sensor performance. After the functionalization of the fiber surface using a methacrylic acid/methacrylate copolymer, an antibody/antigen (IgG/anti-IgG) assay was carried out to assess the performance of sol–gel based titania–silica-coated LPGs as biosensors. The analyte concentration was determined from the wavelength shift at the end of the binding process and from the initial binding rate. This is the first time that a sol–gel based titania–silica-coated LPG is proposed as an effective and feasible label-free biosensor. The specificity of the sensor was validated by performing the same model assay after spiking anti-IgG into human serum. With this structured LPG, detection limits of the order of tens of micrograms per liter (10–11 M) are attained.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>26548589</pmid><doi>10.1021/acs.analchem.5b01841</doi><tpages>8</tpages></addata></record> |
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| source | ACS Publications; MEDLINE |
| subjects | Binding Biological Assay Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Copolymers Dip coatings Fibers Humans Immunoglobulins - blood LPG Reproducibility of Results Sensitivity and Specificity Sensors Silica Silica Gel - chemistry Sol gel process Surface Properties Thin films Titanium - chemistry Viscosity |
| title | Sol–Gel-Based Titania–Silica Thin Film Overlay for Long Period Fiber Grating-Based Biosensors |
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