Paraburkholderia caffeinitolerans sp. nov., a caffeine degrading species isolated from a tea plantation soil sample

A Gram-stain negative, facultatively anaerobic, non-sporulating, non-motile and rod-shaped bacterium, designated CF3 T , was isolated from a tea plantation soil sample and its taxonomic position was determined using polyphasic taxonomy. Strain CF3 T displayed optimum growth at 25 °C, pH 5.0 and in the presence of 0–1 % NaCl. Comparative phylogenetic analysis of the 16S rRNA, recA and gyrB gene sequences showed that the isolate belongs to the genus Paraburkholderia , showing high levels of similarity with respect to Paraburkholderia oxyphila OX-01 T (98.3, 95 and 93 %, respectively) and Paraburkholderia sacchari IPT101 T (98.2, 95 and 95 %, respectively). The predominant ubiquinone was determined to be Q-8, and the polar lipids are phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified amino-phospholipid, three unidentified amino-lipids and three unidentified polar lipids. The major fatty acids were found to be C16:0, C17:0 cyclo, summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The DNA G+C content was found to be 63.8 mol% and the DNA–DNA relatedness values between strain CF3 T and its two close relatives P. oxyphila OX-01 T and P. sacchari IPT101 T was 41 and 40 %, respectively. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate represents a novel species of the genus Paraburkholderia , for which the name Paraburkholderia caffeinitolerans sp. nov. is proposed. The type strain is CF3 T (= LMG 28688 T = CGMCC 1.15105 T ).