Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

Highlights • Atmospheric oxygen and individual culture are standard embryo culture conditions, the effect of combining them is investigated here. • Cleavage timings, hatching rates and blastocyst cell numbers were reduced by either individual culture or atmospheric oxygen. • The combination of indiv...

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Veröffentlicht in:	Reproductive biomedicine online 2016-11, Vol.33 (5), p.537-549
Hauptverfasser:	Kelley, Rebecca L, Gardner, David K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blastocyst - chemistry Blastocyst - cytology Blastocyst - physiology Cellular Microenvironment Cleavage Stage, Ovum - cytology Cleavage Stage, Ovum - physiology Embryo Culture Techniques Embryonic Development group culture IVF Mice Mice, Inbred C57BL Obstetrics and Gynecology oxidative stress Oxygen - chemistry paracrine factors single culture time-lapse Time-Lapse Imaging
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Zusammenfassung:	Highlights • Atmospheric oxygen and individual culture are standard embryo culture conditions, the effect of combining them is investigated here. • Cleavage timings, hatching rates and blastocyst cell numbers were reduced by either individual culture or atmospheric oxygen. • The combination of individual culture and atmospheric oxygen was more detrimental than either treatment alone.
ISSN:	1472-6483 1472-6491
DOI:	10.1016/j.rbmo.2016.08.003