Biodegradation and utilization of dimethylformamide by biofilm forming Paracoccus sp. strains MKU1 and MKU2

[Display omitted] •Paracoccus sp. MKU1 and MKU2 could use DMF as sole carbon and nitrogen source.•MKU1 and MKU2 could form biofilm during DMF utilization.•MKU1 and MKU2 degrade 55% and 46% of the initially supplied DMF (1% v/v).•Biofilm formation is highest with acetate during DMF utilization by Paracoccus sp.•MKU1 has maximum DMF degradation with acetate and MKU2 has with glucose and acetate. Two bacterial strains capable of degrading N,N-dimethylformamide (DMF) were isolated from the effluent and sludge samples of textile and tyre industries. The 16S rRNA gene analysis revealed that bacterial strains belonged to the genera Paracoccus and named as Paracoccus sp. MKU1 and Paracoccus sp. MKU2. The DMF degradation experiments conducted at a DMF concentration of 1% v/v and HPLC analysis revealed that MKU1 and MKU2 degraded 55% and 46% of DMF after 120h of growth. Biofilm quantification by microtiter plate assay revealed that both the bacterial isolates can form efficient biofilm on during DMF utilization. The presence of secondary carbon sources influenced the DMF degradation and biofilm formation where highest biofilm formation was observed in the presence of acetate and enhanced the DMF degradation to a maximum of 86.59% with MKU1 whereas glucose and acetate enhanced DMF degradation by MKU2 to a maximum of 82.7% and 80% respectively.