Transgenic Indian mustard (Brassica juncea) with resistance to the mustard aphid (Lipaphis erysimi Kalt.)

Wheat germ agglutinin (WGA), the chitin-binding lectin from wheat germ, has been shown to be antimetabolic, antifeedant and insecticidal to the mustard aphid (Lipaphis erysimi. Kalt). A cDNA encoding WGA was transferred to Indian mustard (Brassica juncea cv. RLM-198) through Agrobacterium-mediated transformation. Southern analysis of the transgenics showed the integration of the transgene, while Northern and Western analyses demonstrated that the transgene was expressed in the transgenics. Bioassays using leaf discs showed that feeding on transgenics induced high mortality and significantly reduced fecundity of aphids.