Quantitative analysis of amino acids and acylcarnitines combined with untargeted metabolomics using ultra-high performance liquid chromatography and quadrupole time-of-flight mass spectrometry

•We developed a hybrid targeted/untargeted metabolomic method using UHPLC-ESI-QTOF.•The method was fully validated for quantification for eight amino acids and eight acylcarnitines.•Trueness of the amino acids results were all

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Bibliographische Detailangaben
Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2016-08, Vol.1027, p.40-49
Hauptverfasser:	Roy, Cynthia, Tremblay, Pierre-Yves, Bienvenu, Jean-François, Ayotte, Pierre
Format:	Artikel
Sprache:	eng
Schlagworte:	Acylcarnitines Aged Amino acids Amino Acids - blood Amino Acids - metabolism Biomarkers Biomarkers - blood Biomarkers - metabolism Carnitine - analogs & derivatives Carnitine - blood Carnitine - metabolism Chromatography, High Pressure Liquid - methods Epidemiology Female Humans Limit of Detection Liquid chromatography Male Mass spectrometry Metabolites Metabolomics Metabolomics - methods Method validation Middle Aged Plasma Quadrupoles Spectrometry, Mass, Electrospray Ionization - methods UHPLC-ESI-QTOFMS
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Beschreibung
Zusammenfassung:	•We developed a hybrid targeted/untargeted metabolomic method using UHPLC-ESI-QTOF.•The method was fully validated for quantification for eight amino acids and eight acylcarnitines.•Trueness of the amino acids results were all
ISSN:	1570-0232 1873-376X
DOI:	10.1016/j.jchromb.2016.05.006