Preferential expression of domain cassettes 4, 8 and 13 of Plasmodium falciparum erythrocyte membrane protein 1 in severe malaria imported in France

Severe Plasmodium falciparum malaria (SM) involves cytoadhesion of parasitized red blood cells, mediated by P. falciparum erythrocyte membrane protein 1, which is encoded by var genes. Expression of var gene group A and B or encoding domain cassettes DC4, DC5, DC8 and DC13 has been implicated in SM...

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Veröffentlicht in:	Clinical microbiology and infection 2017-03, Vol.23 (3), p.211.e1-211.e4
Hauptverfasser:	Argy, N., Bertin, G.I., Milet, J., Hubert, V., Clain, J., Cojean, S., Houzé, P., Tuikue-Ndam, N., Kendjo, E., Deloron, P., Houzé, S., Matheron, S., Casalino, E., Wolff, M., Delaval, A., Agnamey, P., Durand, R., Pilo, J.E., Rapp, C., Faucher, J.F., Cuisenier, B., Poilane, I., Bemba, D., Roide, A., Debourgogne, A., Thibault, M., Toubas, D., Patoz, P., De Gentile, L., Pons, D., Hurst, J.P., Lohmann, C., Bigel, M.L., Godineau, N., Thouvenin, M., Dunand, J., Ait-Ammar, N., Angoulvant, A., Dahane, N., Lefevre, M., Murat, J.B., Garnaud, C., Dannaoui, E., Botterel, F., Dutoit, E., Dardé, M.L., Ichou, H., Branger, C., Penn, P., Angebault, C., Morio, F., Bret, L., Thellier, M., Mouri, O., Cateau, E., Siriez, J.Y., Fenneteau, O., Revest, M., Belaz, S., Belkadi, G., Hamane, S., Bretagne, S., Aboubacar, A., Leloup, G., Develoux, M., Lapillonne, H., Eloy, O., Nevez, G., Raffenot, D., Buret, B., Desoubeaux, G., Goepp, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult DC13 DC4 DC8 Domain cassette Female France Gene Expression Profiling Humans Imported malaria Malaria, Falciparum - parasitology Malaria, Falciparum - pathology Male Middle Aged Plasmodium falciparum - genetics Plasmodium falciparum - isolation & purification Protozoan Proteins - biosynthesis Protozoan Proteins - genetics Real-Time Polymerase Chain Reaction Severe malaria Severity of Illness Index Var gene Young Adult
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Zusammenfassung:	Severe Plasmodium falciparum malaria (SM) involves cytoadhesion of parasitized red blood cells, mediated by P. falciparum erythrocyte membrane protein 1, which is encoded by var genes. Expression of var gene group A and B or encoding domain cassettes DC4, DC5, DC8 and DC13 has been implicated in SM in African children, but no data exist in the context of imported malaria. The aim of this study was to investigate var gene expression linked to clinical presentation and host factors in SM imported into France. Expression level of var gene groups A, B, C, var1, var2csa, var3 and var genes encoding DC4, DC5, DC8 and DC13 was measured by quantitative RT-PCR and expressed in transcript units. Seventy SM and 48 uncomplicated malaria (UM) P. falciparum cases were analysed according to disease severity, epidemiological characteristics (migrants or travellers) and anti-P. falciparum antibodies. Cluster analysis was performed to identify gene expression profiles. Var1 and B/C expression were higher in UM than SM (0.66 (0–1.1) and 1.88 (1.3–2.4); p
ISSN:	1198-743X 1469-0691
DOI:	10.1016/j.cmi.2016.10.012