Ensuring oxygenation of carrot cell cultures in a Couette viscometer during investigation of shear effects

Mathematical simulation and experimental measurement of dissolved O^sub 2^ were performed for extended (up to 8 h) shear testing of Daucus carota (carrot) cell cultures in a conventional Couette viscometer (0.625 mm annulus). The results suggest O^sub 2^ depletion below critical levels for cell growth may occur. A novel design modification incorporating an O^sub 2^-permeable silicone-layer spun cast on a porous ceramic bowl was devised. It significantly improved oxygenation of the cell cultures, keeping dissolved O^sub 2^ near saturation.[PUBLICATION ABSTRACT]