Phytoremediation of agriculture runoff by filamentous algae poly-culture for biomethane production, and nutrient recovery for secondary cultivation of lipid generating microalgae

[Display omitted] •Natural poly-culture of filamentous algae was grown in agricultural stormwater.•Filamentous algae biomass lipid content was below 2%.•Anaerobic digestion of whole filamentous algae or LEA yielded ∼0.2LCH4pergVS.•Macro- and micronutrients captured from stormwater were released into AD effluent.•AD effluent was successfully used for growth of lipid-accumulating C. sorokiniana. An integrated system was implemented for water phytoremediation and biofuel production through sequential cultivation of filamentous algae followed by cultivation of lipid-producing microalgae Chlorella sorokiniana. Natural poly-culture of filamentous algae was grown in agricultural stormwater using the Algal Turf Scrubber®, harvested and subjected for lipid extraction and/or methane production using anaerobic digestion (AD). While filamentous algae lipid content was too low for feasible biodiesel production (