Elevated IL‐17A and IL‐22 regulate expression of inducible CD38 and Zap‐70 in chronic lymphocytic leukemia

Background In this study, we investigated the role and expression of interleukin (IL)−17A and IL‐22 in chronic lymphocytic leukemia. Methods We evaluated the expression of markers above on CLL by ELISA, qRT‐PCR, flow cytometric analysis and nonparametric Kruskal–Wallis test. Results Quantitative RT‐...

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Veröffentlicht in:	Cytometry. Part B, Clinical cytometry Clinical cytometry, 2018-01, Vol.94 (1), p.143-147
Hauptverfasser:	Kouzegaran, Samaneh, Siroosbakht, Soheila, Farsad, Bahram Fariborz, Rezakhaniha, Bijan, Dormanesh, Banafshe, Behnod, Vahid, Tanha, Amir Saber
Format:	Artikel
Sprache:	eng
Schlagworte:	ADP-ribosyl Cyclase 1 - metabolism Adult Aged CD38 antigen Chronic lymphocytic leukemia Cytometry ELISA Enzyme-linked immunosorbent assay Female Flow cytometry Flow Cytometry - methods Humans Interleukin 22 Interleukin-17 - metabolism interleukins Interleukins - metabolism Leukemia Leukemia, Lymphocytic, Chronic, B-Cell - metabolism Lymphatic leukemia Male Membrane Glycoproteins - metabolism Middle Aged mRNA Patients plasma Polymerase chain reaction QRT‐PCR Serum levels Up-Regulation - physiology ZAP-70 protein ZAP-70 Protein-Tyrosine Kinase - metabolism
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Zusammenfassung:	Background In this study, we investigated the role and expression of interleukin (IL)−17A and IL‐22 in chronic lymphocytic leukemia. Methods We evaluated the expression of markers above on CLL by ELISA, qRT‐PCR, flow cytometric analysis and nonparametric Kruskal–Wallis test. Results Quantitative RT‐PCR revealed that the mRNA levels of IL‐17A and IL‐22 in PBMCs of CLL patients were upregulated compared with those from healthy subjects (mean ± SD: 1.96 ± 0.232 vs.0.72 ± 0.15, P
ISSN:	1552-4949 1552-4957
DOI:	10.1002/cyto.b.21487