Enzyme-linked immunosorbent assays for detection of anti-transcriptional intermediary factor-1 gamma and anti-Mi-2 autoantibodies in dermatomyositis

Highlights • Anti-TIF1γ and anti-Mi-2β Ab ELISAs using a baculovirus expression system were developed and evaluated in a multicenter study. • Anti-TIF1γ and anti-Mi-2β Ab ELISAs showed high sensitivity and specificity demonstrating their utility as easier detection systems for routine testing. • Ant...

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Veröffentlicht in:	Journal of dermatological science 2016-12, Vol.84 (3), p.272-281
Hauptverfasser:	Fujimoto, Manabu, MD, Murakami, Akihiro, Kurei, Shunsuke, Okiyama, Naoko, Kawakami, Atsushi, Mishima, Michiaki, Sato, Shinji, Seishima, Mariko, Suda, Takafumi, Mimori, Tsuneyo, Takehara, Kazuhiko, Kuwana, Masataka
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aged Autoantibodies Autoantibody Cysteine - chemistry Dermatology Dermatomyositis Dermatomyositis - blood Dermatomyositis - immunology Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Female Humans Male Mi-2 Middle Aged Protein Conformation Protein Domains Reproducibility of Results ROC Curve Sensitivity and Specificity Transcription Factors - immunology Transcriptional intermediary factor 1
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Zusammenfassung:	Highlights • Anti-TIF1γ and anti-Mi-2β Ab ELISAs using a baculovirus expression system were developed and evaluated in a multicenter study. • Anti-TIF1γ and anti-Mi-2β Ab ELISAs showed high sensitivity and specificity demonstrating their utility as easier detection systems for routine testing. • Anti-Mi-2β Ab cross-reacts with TIF1γ through the conformational structure of TIF1γ in a cysteine residue-dependent manner due to homologous amino acid sequences within PHD.
ISSN:	0923-1811 1873-569X
DOI:	10.1016/j.jdermsci.2016.09.013