New and effective entrapment of polyelectrolyte-enzyme-complexes in Lentikats

Amyloglucosidase EC 3.2.1.3 was used as model for the immobilisation of enzymes in poly(vinylalcohol) hydrogel (LentiKats) lenses. The entrapment of the enzyme in PVA-hydrogel based on a two-step procedure, firstly its coupling to polyelectrolytes increased the structure level of the enzyme, and sub...

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Veröffentlicht in:	Biotechnology letters 2001-08, Vol.23 (16), p.1303-1307
Hauptverfasser:	CZICHOCKI, Gunther, DAUTZENBERG, Herbert, CAPAN, Emine, VORLOP, Klaus-Dieter
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Enzymes Fundamental and applied biological sciences. Psychology Hydrogels Immobilization of enzymes and other molecules Immobilization techniques Methods. Procedures. Technologies Polyelectrolytes polyvinyl alcohol
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Zusammenfassung:	Amyloglucosidase EC 3.2.1.3 was used as model for the immobilisation of enzymes in poly(vinylalcohol) hydrogel (LentiKats) lenses. The entrapment of the enzyme in PVA-hydrogel based on a two-step procedure, firstly its coupling to polyelectrolytes increased the structure level of the enzyme, and subsequently, the resulting complex was entrapped in LentiKats. The immobilised enzyme retained 45% of its original activity and lost no activity over five repeated batch runs.[PUBLICATION ABSTRACT]
ISSN:	0141-5492 1573-6776
DOI:	10.1023/A:1010569322537