Extraction of Benzoylecgonine from Urine Specimens with Cerex Polycrom™ Clin II Solid-Phase Extraction Columns and the Speedisk™ Pressure Processor
Benzoylecgonine (BZE) extraction from urine was explored using Cerex Polycrom Clin II solid-phase extraction (SPE) columns and the Speedisk 48 Pressure Processor as an alternative to the Prep™1 automated sample processor and XTRX™ Type RP/W columns. Linearity for urine standards extracted using the...
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| Veröffentlicht in: | Journal of analytical toxicology 2001-10, Vol.25 (7), p.616-620 |
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| creator | Kuhnle, Judith A. Churley, Melissa Kawasaki, Shiralen Y. Lyons, Timothy P. Bruins, Mark R. |
| description | Benzoylecgonine (BZE) extraction from urine was explored using Cerex Polycrom Clin II solid-phase extraction (SPE) columns and the Speedisk 48 Pressure Processor as an alternative to the Prep™1 automated sample processor and XTRX™ Type RP/W columns. Linearity for urine standards extracted using the Cerex-Speedisk method ranged from 20 to 3000 ng/mL. The mean recovery at the 100-ng/mL cutoff for three lots of columns was 92%. The mean of the within-run means for three batches, which had coefficients of variations of 1.8% or less, was 101.3 ng/mL at the lO0-ng/mL cutoff level. Forty-six specimens known to contain BZE were analyzed by both the Prep1-Type RP/W and Cerex-Speedisk methods. The correlation for specimen BZE concentrations between the two methods gave an r2 of 0.9999 and a slope of 1.03. The Cerex-Speedisk system is an inexpensive alternative to the Prep1-Type RP/W system. It is less costly, requires little maintenance, has a small footprint, is hood compatible, and can process four times the number of specimens in a given time. |
| doi_str_mv | 10.1093/jat/25.7.616 |
| format | Article |
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Linearity for urine standards extracted using the Cerex-Speedisk method ranged from 20 to 3000 ng/mL. The mean recovery at the 100-ng/mL cutoff for three lots of columns was 92%. The mean of the within-run means for three batches, which had coefficients of variations of 1.8% or less, was 101.3 ng/mL at the lO0-ng/mL cutoff level. Forty-six specimens known to contain BZE were analyzed by both the Prep1-Type RP/W and Cerex-Speedisk methods. The correlation for specimen BZE concentrations between the two methods gave an r2 of 0.9999 and a slope of 1.03. The Cerex-Speedisk system is an inexpensive alternative to the Prep1-Type RP/W system. It is less costly, requires little maintenance, has a small footprint, is hood compatible, and can process four times the number of specimens in a given time.</description><identifier>ISSN: 0146-4760</identifier><identifier>EISSN: 1945-2403</identifier><identifier>DOI: 10.1093/jat/25.7.616</identifier><identifier>PMID: 11599610</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Automation ; benzoylecgonine ; Chromatography, Ion Exchange - methods ; Cocaine - analogs & derivatives ; Cocaine - urine ; Cocaine-Related Disorders - diagnosis ; Humans ; Particle Size ; Sensitivity and Specificity ; Substance Abuse Detection - methods</subject><ispartof>Journal of analytical toxicology, 2001-10, Vol.25 (7), p.616-620</ispartof><rights>2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-c64fbdfbfb79de3a7aeb172043d379711397df1cde77a421da71296f9e587363</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11599610$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kuhnle, Judith A.</creatorcontrib><creatorcontrib>Churley, Melissa</creatorcontrib><creatorcontrib>Kawasaki, Shiralen Y.</creatorcontrib><creatorcontrib>Lyons, Timothy P.</creatorcontrib><creatorcontrib>Bruins, Mark R.</creatorcontrib><title>Extraction of Benzoylecgonine from Urine Specimens with Cerex Polycrom™ Clin II Solid-Phase Extraction Columns and the Speedisk™ Pressure Processor</title><title>Journal of analytical toxicology</title><addtitle>Journal of Analytical Toxicology</addtitle><addtitle>Journal of Analytical Toxicology</addtitle><description>Benzoylecgonine (BZE) extraction from urine was explored using Cerex Polycrom Clin II solid-phase extraction (SPE) columns and the Speedisk 48 Pressure Processor as an alternative to the Prep™1 automated sample processor and XTRX™ Type RP/W columns. Linearity for urine standards extracted using the Cerex-Speedisk method ranged from 20 to 3000 ng/mL. The mean recovery at the 100-ng/mL cutoff for three lots of columns was 92%. The mean of the within-run means for three batches, which had coefficients of variations of 1.8% or less, was 101.3 ng/mL at the lO0-ng/mL cutoff level. Forty-six specimens known to contain BZE were analyzed by both the Prep1-Type RP/W and Cerex-Speedisk methods. The correlation for specimen BZE concentrations between the two methods gave an r2 of 0.9999 and a slope of 1.03. The Cerex-Speedisk system is an inexpensive alternative to the Prep1-Type RP/W system. It is less costly, requires little maintenance, has a small footprint, is hood compatible, and can process four times the number of specimens in a given time.</description><subject>Automation</subject><subject>benzoylecgonine</subject><subject>Chromatography, Ion Exchange - methods</subject><subject>Cocaine - analogs & derivatives</subject><subject>Cocaine - urine</subject><subject>Cocaine-Related Disorders - diagnosis</subject><subject>Humans</subject><subject>Particle Size</subject><subject>Sensitivity and Specificity</subject><subject>Substance Abuse Detection - methods</subject><issn>0146-4760</issn><issn>1945-2403</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGO0zAQhi0EYsvCjTPyCS6ka8eOXR8hWthqK1HUIhAXy4kn1LtJXOxEtJx5B-48Gk-CSyvgxmn-wzefNPMj9JiSKSWKXdyY4SIvpnIqqLiDJlTxIss5YXfRhFAuMi4FOUMPYrwhhIqZYPfRGaWFUoKSCfp-uRuCqQfne-wb_BL6r37fQv3J964H3ATf4XfhEFdbqF0HfcRf3LDBJQTY4aVv93Vifn77gcvW9Xg-xyvfOpstNyYC_sde-nbs0rbpLR42v31gXbw9rC4DxDgGSMHXKfrwEN1rTBvh0Wmeo_Wry3V5lS3evJ6XLxZZzXMxZLXgTWWbqqmkssCMNFBRmRPOLJNKUsqUtA2tLUhpeE6tkTRXolFQzCQT7Bw9PWq3wX8eIQ66c7GGtjU9-DFqOsuV5Jwm8PkRTNfGGKDR2-A6E_aaEn3oQacedF5oqVMPCX9y8o5VB_YvfHp8Ap4dAT9u_6fKjqSLA-z-sCbcaiGZLPTVh496sV6t3q6vr_V79gsQiqTn</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>Kuhnle, Judith A.</creator><creator>Churley, Melissa</creator><creator>Kawasaki, Shiralen Y.</creator><creator>Lyons, Timothy P.</creator><creator>Bruins, Mark R.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20011001</creationdate><title>Extraction of Benzoylecgonine from Urine Specimens with Cerex Polycrom™ Clin II Solid-Phase Extraction Columns and the Speedisk™ Pressure Processor</title><author>Kuhnle, Judith A. ; Churley, Melissa ; Kawasaki, Shiralen Y. ; Lyons, Timothy P. ; Bruins, Mark R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-c64fbdfbfb79de3a7aeb172043d379711397df1cde77a421da71296f9e587363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Automation</topic><topic>benzoylecgonine</topic><topic>Chromatography, Ion Exchange - methods</topic><topic>Cocaine - analogs & derivatives</topic><topic>Cocaine - urine</topic><topic>Cocaine-Related Disorders - diagnosis</topic><topic>Humans</topic><topic>Particle Size</topic><topic>Sensitivity and Specificity</topic><topic>Substance Abuse Detection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuhnle, Judith A.</creatorcontrib><creatorcontrib>Churley, Melissa</creatorcontrib><creatorcontrib>Kawasaki, Shiralen Y.</creatorcontrib><creatorcontrib>Lyons, Timothy P.</creatorcontrib><creatorcontrib>Bruins, Mark R.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of analytical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuhnle, Judith A.</au><au>Churley, Melissa</au><au>Kawasaki, Shiralen Y.</au><au>Lyons, Timothy P.</au><au>Bruins, Mark R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extraction of Benzoylecgonine from Urine Specimens with Cerex Polycrom™ Clin II Solid-Phase Extraction Columns and the Speedisk™ Pressure Processor</atitle><jtitle>Journal of analytical toxicology</jtitle><stitle>Journal of Analytical Toxicology</stitle><addtitle>Journal of Analytical Toxicology</addtitle><date>2001-10-01</date><risdate>2001</risdate><volume>25</volume><issue>7</issue><spage>616</spage><epage>620</epage><pages>616-620</pages><issn>0146-4760</issn><eissn>1945-2403</eissn><abstract>Benzoylecgonine (BZE) extraction from urine was explored using Cerex Polycrom Clin II solid-phase extraction (SPE) columns and the Speedisk 48 Pressure Processor as an alternative to the Prep™1 automated sample processor and XTRX™ Type RP/W columns. Linearity for urine standards extracted using the Cerex-Speedisk method ranged from 20 to 3000 ng/mL. The mean recovery at the 100-ng/mL cutoff for three lots of columns was 92%. The mean of the within-run means for three batches, which had coefficients of variations of 1.8% or less, was 101.3 ng/mL at the lO0-ng/mL cutoff level. Forty-six specimens known to contain BZE were analyzed by both the Prep1-Type RP/W and Cerex-Speedisk methods. The correlation for specimen BZE concentrations between the two methods gave an r2 of 0.9999 and a slope of 1.03. The Cerex-Speedisk system is an inexpensive alternative to the Prep1-Type RP/W system. It is less costly, requires little maintenance, has a small footprint, is hood compatible, and can process four times the number of specimens in a given time.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>11599610</pmid><doi>10.1093/jat/25.7.616</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of analytical toxicology, 2001-10, Vol.25 (7), p.616-620 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Automation benzoylecgonine Chromatography, Ion Exchange - methods Cocaine - analogs & derivatives Cocaine - urine Cocaine-Related Disorders - diagnosis Humans Particle Size Sensitivity and Specificity Substance Abuse Detection - methods |
| title | Extraction of Benzoylecgonine from Urine Specimens with Cerex Polycrom™ Clin II Solid-Phase Extraction Columns and the Speedisk™ Pressure Processor |
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