Molecular cloning, characterization and induced expression analysis of ion channel gene RSC 1 in Rhopalosiphum padi(Hemiptera: Aphididae)

[Aim] SC1 (sodium channel 1) is an important ion channel in arthropods including insects. It is considered as a possible new neuro target. This study aims to investigate the SC1 gene from Rhopalosiphum padi, and to analyze the primary biological functions of the gene and the evolutionary relationship of SC1 channels with voltage-gated sodium channel and voltage-gated calcium channel. [Methods] RT-PCR technique was used to clone the full-length ORF of RC1 gene from R. padi. After R. padi adults were exposed to different concentrations of [lambda]-cyhalothrin for 6 and 24 h, respectively, the expression levels of SC1 were analyzed by real-time quantitative PCR. [Results] The ORF of RSC 1 (GenBank accession no: KU640190) is 6 687 bp in length with the deduced amino acid sequence of 2 228 residues. Structural analysis showed that the RSC1 has the typical structural feature of SC1 channel, and contains a unique DEEA motif, which is different from the motif of sodium channel and calcium channel. Phylogenetic analys