Microwave-assisted extraction of polysaccharides from Cyphomandra betacea and its biological activities
•Microwave-assisted the extraction of polysaccharides from Cyphomandra betacea.•Optimization was carried out for the isolation of polysaccharides from Cyphomandra betacea using RSM.•This Polysaccharides revealed better antioxidant activity and anti-cancer activity. Response Surface Methodology (RSM)...
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| Veröffentlicht in: | International journal of biological macromolecules 2016-11, Vol.92, p.682-693 |
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| container_title | International journal of biological macromolecules |
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| creator | C, Senthil Kumar M, Sivakumar K, Ruckmani |
| description | •Microwave-assisted the extraction of polysaccharides from Cyphomandra betacea.•Optimization was carried out for the isolation of polysaccharides from Cyphomandra betacea using RSM.•This Polysaccharides revealed better antioxidant activity and anti-cancer activity.
Response Surface Methodology (RSM) was used to optimize the parameters for microwave-assisted extraction of polysaccharides from Cyphomandra betacea. The results showed a good fit with a second-order polynomial equation that was statistically acceptable at P |
| doi_str_mv | 10.1016/j.ijbiomac.2016.07.062 |
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Response Surface Methodology (RSM) was used to optimize the parameters for microwave-assisted extraction of polysaccharides from Cyphomandra betacea. The results showed a good fit with a second-order polynomial equation that was statistically acceptable at P<0.05. Optimal conditions for the extraction of polysaccharides were: extraction time, 2h; microwave power, 400W; extraction temperature, 60°C; and ratio of raw material to water 1:40 (g/mL). Under the optimized conditions, the yield of polysaccharides was found to be relatively high (about 36.52%). The in vitro biological activities of antioxidant and antitumor were evaluated. The IC50 value of polysaccharides was found to be 3mg/mL. The percentage of Cell viability was determined by MTT assay. Our results showed that polysaccharides inhibited proliferation of MCF-7 (Breast carcinoma), A549 (Human lung carcinoma) and HepG2 (Liver carcinoma) with an IC50 of 0.23mg/mL, 0.17mg/mL and 0.62mg/mL respectively after 48h incubation. Polysaccharides were shown to promote apoptosis as seen in the nuclear morphological examination study using acridine orange (AO) and ethidium bromide (EB) staining. This is the first report on the effects of polysaccharides extracted from Cyphomandra betacea which exhibited stronger antioxidant and antitumor activities.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2016.07.062</identifier><identifier>PMID: 27456125</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Analysis of Variance ; Antineoplastic Agents - pharmacology ; Antioxidant ; Antioxidants - pharmacology ; Antitumor ; Apoptosis - drug effects ; Cell Line, Tumor ; Cell Shape - drug effects ; Chromatography, Liquid ; Cyphomandra betacea ; Extraction ; Humans ; Microwave ; Microwaves ; Models, Theoretical ; Optimisation ; Polysaccharides ; Polysaccharides - chemistry ; Polysaccharides - isolation & purification ; Polysaccharides - pharmacology ; Reproducibility of Results ; RSM ; Solanum - chemistry ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry ; Temperature ; Time Factors ; Water - chemistry</subject><ispartof>International journal of biological macromolecules, 2016-11, Vol.92, p.682-693</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-179ada523b0bcd5748c661273d47d0ed3ba0327441178826ca6190b195c1827d3</citedby><cites>FETCH-LOGICAL-c434t-179ada523b0bcd5748c661273d47d0ed3ba0327441178826ca6190b195c1827d3</cites><orcidid>0000-0003-4125-0244</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2016.07.062$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27456125$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>C, Senthil Kumar</creatorcontrib><creatorcontrib>M, Sivakumar</creatorcontrib><creatorcontrib>K, Ruckmani</creatorcontrib><title>Microwave-assisted extraction of polysaccharides from Cyphomandra betacea and its biological activities</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>•Microwave-assisted the extraction of polysaccharides from Cyphomandra betacea.•Optimization was carried out for the isolation of polysaccharides from Cyphomandra betacea using RSM.•This Polysaccharides revealed better antioxidant activity and anti-cancer activity.
Response Surface Methodology (RSM) was used to optimize the parameters for microwave-assisted extraction of polysaccharides from Cyphomandra betacea. The results showed a good fit with a second-order polynomial equation that was statistically acceptable at P<0.05. Optimal conditions for the extraction of polysaccharides were: extraction time, 2h; microwave power, 400W; extraction temperature, 60°C; and ratio of raw material to water 1:40 (g/mL). Under the optimized conditions, the yield of polysaccharides was found to be relatively high (about 36.52%). The in vitro biological activities of antioxidant and antitumor were evaluated. The IC50 value of polysaccharides was found to be 3mg/mL. The percentage of Cell viability was determined by MTT assay. Our results showed that polysaccharides inhibited proliferation of MCF-7 (Breast carcinoma), A549 (Human lung carcinoma) and HepG2 (Liver carcinoma) with an IC50 of 0.23mg/mL, 0.17mg/mL and 0.62mg/mL respectively after 48h incubation. Polysaccharides were shown to promote apoptosis as seen in the nuclear morphological examination study using acridine orange (AO) and ethidium bromide (EB) staining. This is the first report on the effects of polysaccharides extracted from Cyphomandra betacea which exhibited stronger antioxidant and antitumor activities.</description><subject>Analysis of Variance</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Antioxidant</subject><subject>Antioxidants - pharmacology</subject><subject>Antitumor</subject><subject>Apoptosis - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Shape - drug effects</subject><subject>Chromatography, Liquid</subject><subject>Cyphomandra betacea</subject><subject>Extraction</subject><subject>Humans</subject><subject>Microwave</subject><subject>Microwaves</subject><subject>Models, Theoretical</subject><subject>Optimisation</subject><subject>Polysaccharides</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - isolation & purification</subject><subject>Polysaccharides - pharmacology</subject><subject>Reproducibility of Results</subject><subject>RSM</subject><subject>Solanum - chemistry</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tandem Mass Spectrometry</subject><subject>Temperature</subject><subject>Time Factors</subject><subject>Water - chemistry</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PAyEQhonRaP34Cw1HL7vCsgvbm6bxK9F40TOZhaml2S0VaLX_XpqqV5NJJkPemfflIWTMWckZl1eL0i065wcwZZXnkqmSyeqAjHirJgVjTBySEeM1L1ou2Ak5jXGRX2XD22NyUqm6kbxqRuT92ZngP2GDBcToYkJL8SsFMMn5JfUzuvL9NoIxcwjOYqSz4Ac63a7m2XxpA9AOExgEmifqUqQ5Vu_fnYGe7q5sXHIYz8nRDPqIFz_9jLzd3b5OH4qnl_vH6c1TYWpRp4KrCVhoKtGxzthG1a2ROagStlaWoRUdMJHT15yrtq2kAcknrOOTxvC2Ulackcv93VXwH2uMSQ8uGux7WKJfR51VUuUSVZbKvTQDiDHgTK-CGyBsNWd6B1kv9C9kvYOsmdIZcl4c_3isuwHt39ov1Sy43gsw_3TjMOhoHC4NWhfQJG29-8_jGyVSkhc</recordid><startdate>201611</startdate><enddate>201611</enddate><creator>C, Senthil Kumar</creator><creator>M, Sivakumar</creator><creator>K, Ruckmani</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4125-0244</orcidid></search><sort><creationdate>201611</creationdate><title>Microwave-assisted extraction of polysaccharides from Cyphomandra betacea and its biological activities</title><author>C, Senthil Kumar ; M, Sivakumar ; K, Ruckmani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-179ada523b0bcd5748c661273d47d0ed3ba0327441178826ca6190b195c1827d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis of Variance</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Antioxidant</topic><topic>Antioxidants - pharmacology</topic><topic>Antitumor</topic><topic>Apoptosis - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Shape - drug effects</topic><topic>Chromatography, Liquid</topic><topic>Cyphomandra betacea</topic><topic>Extraction</topic><topic>Humans</topic><topic>Microwave</topic><topic>Microwaves</topic><topic>Models, Theoretical</topic><topic>Optimisation</topic><topic>Polysaccharides</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - isolation & purification</topic><topic>Polysaccharides - pharmacology</topic><topic>Reproducibility of Results</topic><topic>RSM</topic><topic>Solanum - chemistry</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Tandem Mass Spectrometry</topic><topic>Temperature</topic><topic>Time Factors</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>C, Senthil Kumar</creatorcontrib><creatorcontrib>M, Sivakumar</creatorcontrib><creatorcontrib>K, Ruckmani</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>C, Senthil Kumar</au><au>M, Sivakumar</au><au>K, Ruckmani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microwave-assisted extraction of polysaccharides from Cyphomandra betacea and its biological activities</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2016-11</date><risdate>2016</risdate><volume>92</volume><spage>682</spage><epage>693</epage><pages>682-693</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>•Microwave-assisted the extraction of polysaccharides from Cyphomandra betacea.•Optimization was carried out for the isolation of polysaccharides from Cyphomandra betacea using RSM.•This Polysaccharides revealed better antioxidant activity and anti-cancer activity.
Response Surface Methodology (RSM) was used to optimize the parameters for microwave-assisted extraction of polysaccharides from Cyphomandra betacea. The results showed a good fit with a second-order polynomial equation that was statistically acceptable at P<0.05. Optimal conditions for the extraction of polysaccharides were: extraction time, 2h; microwave power, 400W; extraction temperature, 60°C; and ratio of raw material to water 1:40 (g/mL). Under the optimized conditions, the yield of polysaccharides was found to be relatively high (about 36.52%). The in vitro biological activities of antioxidant and antitumor were evaluated. The IC50 value of polysaccharides was found to be 3mg/mL. The percentage of Cell viability was determined by MTT assay. Our results showed that polysaccharides inhibited proliferation of MCF-7 (Breast carcinoma), A549 (Human lung carcinoma) and HepG2 (Liver carcinoma) with an IC50 of 0.23mg/mL, 0.17mg/mL and 0.62mg/mL respectively after 48h incubation. Polysaccharides were shown to promote apoptosis as seen in the nuclear morphological examination study using acridine orange (AO) and ethidium bromide (EB) staining. This is the first report on the effects of polysaccharides extracted from Cyphomandra betacea which exhibited stronger antioxidant and antitumor activities.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27456125</pmid><doi>10.1016/j.ijbiomac.2016.07.062</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-4125-0244</orcidid></addata></record> |
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| subjects | Analysis of Variance Antineoplastic Agents - pharmacology Antioxidant Antioxidants - pharmacology Antitumor Apoptosis - drug effects Cell Line, Tumor Cell Shape - drug effects Chromatography, Liquid Cyphomandra betacea Extraction Humans Microwave Microwaves Models, Theoretical Optimisation Polysaccharides Polysaccharides - chemistry Polysaccharides - isolation & purification Polysaccharides - pharmacology Reproducibility of Results RSM Solanum - chemistry Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Temperature Time Factors Water - chemistry |
| title | Microwave-assisted extraction of polysaccharides from Cyphomandra betacea and its biological activities |
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